Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22136 | 5' | -54 | NC_004927.1 | + | 21118 | 0.66 | 0.872387 |
Target: 5'- --aGGGAGAUGCgcuccucgaagUCGCCGcucgCGCCg -3' miRNA: 3'- ucgCCUUCUACG-----------AGUGGCucaaGCGG- -5' |
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22136 | 5' | -54 | NC_004927.1 | + | 2106 | 0.66 | 0.864523 |
Target: 5'- uAGCGccGAuGGUGUUCACCGuauucucaacgaAG-UCGCCg -3' miRNA: 3'- -UCGC--CUuCUACGAGUGGC------------UCaAGCGG- -5' |
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22136 | 5' | -54 | NC_004927.1 | + | 16939 | 0.66 | 0.857245 |
Target: 5'- cGGCGGcuGAUGCUCuacaAUCGGGgaacguggcacgacCGCCg -3' miRNA: 3'- -UCGCCuuCUACGAG----UGGCUCaa------------GCGG- -5' |
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22136 | 5' | -54 | NC_004927.1 | + | 59872 | 0.66 | 0.856425 |
Target: 5'- aAGCuGGAGAUGgaUACCGAGgaaGCg -3' miRNA: 3'- -UCGcCUUCUACgaGUGGCUCaagCGg -5' |
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22136 | 5' | -54 | NC_004927.1 | + | 13461 | 0.66 | 0.854778 |
Target: 5'- uGUGGGAGGUGUggguuguaucgaCGCCGAGcUUGUCc -3' miRNA: 3'- uCGCCUUCUACGa-----------GUGGCUCaAGCGG- -5' |
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22136 | 5' | -54 | NC_004927.1 | + | 57136 | 0.66 | 0.851456 |
Target: 5'- uGCGGAacacgacggcguuguGGAuaUGUUCAcuCCGAuUUCGCCu -3' miRNA: 3'- uCGCCU---------------UCU--ACGAGU--GGCUcAAGCGG- -5' |
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22136 | 5' | -54 | NC_004927.1 | + | 13404 | 0.67 | 0.812679 |
Target: 5'- cGGCGGAAca-----GCCGAGUUCGUCu -3' miRNA: 3'- -UCGCCUUcuacgagUGGCUCAAGCGG- -5' |
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22136 | 5' | -54 | NC_004927.1 | + | 7765 | 0.67 | 0.812679 |
Target: 5'- -aCGGAAGuUGCUaCACCGGa--CGCCg -3' miRNA: 3'- ucGCCUUCuACGA-GUGGCUcaaGCGG- -5' |
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22136 | 5' | -54 | NC_004927.1 | + | 41344 | 0.67 | 0.803341 |
Target: 5'- aGGCcG-AGGUGCgcCACCGAGUUCGa- -3' miRNA: 3'- -UCGcCuUCUACGa-GUGGCUCAAGCgg -5' |
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22136 | 5' | -54 | NC_004927.1 | + | 73916 | 0.67 | 0.793829 |
Target: 5'- cGGUGGggGAgaagcgGCgaugaGCCGAGcUUCGaCCc -3' miRNA: 3'- -UCGCCuuCUa-----CGag---UGGCUC-AAGC-GG- -5' |
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22136 | 5' | -54 | NC_004927.1 | + | 25024 | 0.68 | 0.764352 |
Target: 5'- uAGCGGGAGAUuagGUcuUCaACCGAGagCGCg -3' miRNA: 3'- -UCGCCUUCUA---CG--AG-UGGCUCaaGCGg -5' |
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22136 | 5' | -54 | NC_004927.1 | + | 21457 | 0.71 | 0.60502 |
Target: 5'- cAGuuGccGAgGCUCACCGGGUcgUCGCCc -3' miRNA: 3'- -UCgcCuuCUaCGAGUGGCUCA--AGCGG- -5' |
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22136 | 5' | -54 | NC_004927.1 | + | 16324 | 0.72 | 0.540835 |
Target: 5'- gAGCGGAugauugGGAUGCUCcgauGCCGG--UCGUCg -3' miRNA: 3'- -UCGCCU------UCUACGAG----UGGCUcaAGCGG- -5' |
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22136 | 5' | -54 | NC_004927.1 | + | 68399 | 0.76 | 0.342383 |
Target: 5'- cGCGGAAGcgugaGCUUcuuGCUGAGUUCGCUg -3' miRNA: 3'- uCGCCUUCua---CGAG---UGGCUCAAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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