Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22138 | 3' | -56.2 | NC_004927.1 | + | 10405 | 0.66 | 0.802064 |
Target: 5'- gCCGGGAUgaccaacucCCGAcGAcgcCCCACGGuggGGCGu -3' miRNA: 3'- -GGCCCUGa--------GGCUuCU---GGGUGCU---UCGU- -5' |
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22138 | 3' | -56.2 | NC_004927.1 | + | 34918 | 0.66 | 0.754117 |
Target: 5'- gUGGGuCUUCGGAGuCCCgGCGAGcGCGu -3' miRNA: 3'- gGCCCuGAGGCUUCuGGG-UGCUU-CGU- -5' |
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22138 | 3' | -56.2 | NC_004927.1 | + | 38725 | 0.66 | 0.754117 |
Target: 5'- aCGGGAa-CCGAGucGGCCCGCGccGUg -3' miRNA: 3'- gGCCCUgaGGCUU--CUGGGUGCuuCGu -5' |
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22138 | 3' | -56.2 | NC_004927.1 | + | 54769 | 0.67 | 0.713632 |
Target: 5'- gUCGGuGACUCCacGAGuGACCC-CGgcGCGg -3' miRNA: 3'- -GGCC-CUGAGG--CUU-CUGGGuGCuuCGU- -5' |
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22138 | 3' | -56.2 | NC_004927.1 | + | 23141 | 0.67 | 0.7033 |
Target: 5'- gCCGGGcCUCCguGAAGugCUgAgGGAGCGg -3' miRNA: 3'- -GGCCCuGAGG--CUUCugGG-UgCUUCGU- -5' |
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22138 | 3' | -56.2 | NC_004927.1 | + | 68993 | 0.68 | 0.661429 |
Target: 5'- aCGGGcGCUCCGA---CCCACGAcgaaguggugaAGCGc -3' miRNA: 3'- gGCCC-UGAGGCUucuGGGUGCU-----------UCGU- -5' |
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22138 | 3' | -56.2 | NC_004927.1 | + | 72388 | 0.69 | 0.598041 |
Target: 5'- aCCcGGACUCCgcucgugguGAAGACCCucCGAAGaCGg -3' miRNA: 3'- -GGcCCUGAGG---------CUUCUGGGu-GCUUC-GU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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