Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22139 | 5' | -55.8 | NC_004927.1 | + | 26303 | 0.66 | 0.749584 |
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Target: 5'- aCGUCGUAGAGCCaCGUCa-GcgGGAg- -3' miRNA: 3'- -GCGGCAUCUUGG-GCGGagCuaCCUaa -5' |
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| 22139 | 5' | -55.8 | NC_004927.1 | + | 545 | 0.67 | 0.72906 |
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Target: 5'- aCGUgGUAGGuACaaGCCUCGAUGcGGUa -3' miRNA: 3'- -GCGgCAUCU-UGggCGGAGCUAC-CUAa -5' |
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| 22139 | 5' | -55.8 | NC_004927.1 | + | 32352 | 0.68 | 0.611791 |
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Target: 5'- uCGUCGggAGuGACCCGaCUCGAUGGGa- -3' miRNA: 3'- -GCGGCa-UC-UUGGGCgGAGCUACCUaa -5' |
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| 22139 | 5' | -55.8 | NC_004927.1 | + | 74451 | 0.69 | 0.601043 |
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Target: 5'- -aCCG-AGuAGCCCGCCUCGAUGa--- -3' miRNA: 3'- gcGGCaUC-UUGGGCGGAGCUACcuaa -5' |
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| 22139 | 5' | -55.8 | NC_004927.1 | + | 64259 | 0.71 | 0.476181 |
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Target: 5'- aGCCGcuacgGGAAUCCGaCCUcccCGAUGGAUa -3' miRNA: 3'- gCGGCa----UCUUGGGC-GGA---GCUACCUAa -5' |
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| 22139 | 5' | -55.8 | NC_004927.1 | + | 63776 | 0.71 | 0.446904 |
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Target: 5'- uCGCCGUGacACCCaCCUCGAugagUGGAUg -3' miRNA: 3'- -GCGGCAUcuUGGGcGGAGCU----ACCUAa -5' |
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| 22139 | 5' | -55.8 | NC_004927.1 | + | 4641 | 0.72 | 0.397747 |
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Target: 5'- uCGUCGUAGAacuucgcuaccgaaGCCUGCCggagUCGGUGGAg- -3' miRNA: 3'- -GCGGCAUCU--------------UGGGCGG----AGCUACCUaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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