miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22140 5' -50.8 NC_004927.1 + 68021 0.66 0.952219
Target:  5'- aCAGCcCGCUGUUgGCGucCUGAuggaGGACg -3'
miRNA:   3'- -GUCGaGCGAUAGgUGCucGAUU----UCUG- -5'
22140 5' -50.8 NC_004927.1 + 60327 0.67 0.92043
Target:  5'- uUAGCaucgagUCGCuUAUCUACGAuGCUcAGGGCg -3'
miRNA:   3'- -GUCG------AGCG-AUAGGUGCU-CGAuUUCUG- -5'
22140 5' -50.8 NC_004927.1 + 17917 0.7 0.777907
Target:  5'- aGGCUcCGUg--CCACGAGCUGAuguGAUu -3'
miRNA:   3'- gUCGA-GCGauaGGUGCUCGAUUu--CUG- -5'
22140 5' -50.8 NC_004927.1 + 55038 0.75 0.492965
Target:  5'- aAGCUCGCcGUCCACGGuGCgacguguGAGGCu -3'
miRNA:   3'- gUCGAGCGaUAGGUGCU-CGau-----UUCUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.