Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22142 | 5' | -43.5 | NC_004927.1 | + | 56582 | 0.66 | 0.999961 |
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Target: 5'- -gAUUAUcaGCGGCaGAACGUCCGAc-- -3' miRNA: 3'- caUGAUA--UGCUGcUUUGUAGGCUuaa -5' |
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| 22142 | 5' | -43.5 | NC_004927.1 | + | 67128 | 0.67 | 0.9999 |
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Target: 5'- -gACaGUACGGCGcaccgcGCGUCCGAAc- -3' miRNA: 3'- caUGaUAUGCUGCuu----UGUAGGCUUaa -5' |
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| 22142 | 5' | -43.5 | NC_004927.1 | + | 13412 | 0.67 | 0.999867 |
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Target: 5'- -gAUUGUccCGGCGGAACAgCCGAGUUc -3' miRNA: 3'- caUGAUAu-GCUGCUUUGUaGGCUUAA- -5' |
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| 22142 | 5' | -43.5 | NC_004927.1 | + | 49078 | 0.68 | 0.999698 |
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Target: 5'- -gACUAuUugGACGAGAgaacguuccuCGUCCGAAa- -3' miRNA: 3'- caUGAU-AugCUGCUUU----------GUAGGCUUaa -5' |
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| 22142 | 5' | -43.5 | NC_004927.1 | + | 63101 | 0.68 | 0.9996 |
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Target: 5'- -----cUACGACGAAACugcuggaacgacuGUCCGAGUg -3' miRNA: 3'- caugauAUGCUGCUUUG-------------UAGGCUUAa -5' |
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| 22142 | 5' | -43.5 | NC_004927.1 | + | 70292 | 0.68 | 0.999198 |
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Target: 5'- aGUGCggGUGCGAUGAagcuggaaggGACGUUCGAGc- -3' miRNA: 3'- -CAUGa-UAUGCUGCU----------UUGUAGGCUUaa -5' |
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| 22142 | 5' | -43.5 | NC_004927.1 | + | 47236 | 0.69 | 0.998751 |
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Target: 5'- -gACUGgu-GGCGAGGCGUCCGGc-- -3' miRNA: 3'- caUGAUaugCUGCUUUGUAGGCUuaa -5' |
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| 22142 | 5' | -43.5 | NC_004927.1 | + | 5387 | 0.7 | 0.997694 |
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Target: 5'- aUACUcUGCGGCGAAuACAUCCa---- -3' miRNA: 3'- cAUGAuAUGCUGCUU-UGUAGGcuuaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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