Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22144 | 3' | -57.9 | NC_004927.1 | + | 71110 | 0.69 | 0.505197 |
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Target: 5'- -cCCGACGAGG---GCGAGCGGAUGg -3' miRNA: 3'- gcGGCUGCUUCgggCGUUCGCCUACa -5' |
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| 22144 | 3' | -57.9 | NC_004927.1 | + | 68046 | 0.66 | 0.68664 |
|
Target: 5'- cCGCCGACGcaGauucggcaaacgcacAGCCCGCuguuGGCGuccuGAUGg -3' miRNA: 3'- -GCGGCUGC--U---------------UCGGGCGu---UCGC----CUACa -5' |
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| 22144 | 3' | -57.9 | NC_004927.1 | + | 67932 | 0.69 | 0.479473 |
|
Target: 5'- gGCuCGAUGAAGCCuacgcaaggaacgcaCGCAGGCcggGGAUGa -3' miRNA: 3'- gCG-GCUGCUUCGG---------------GCGUUCG---CCUACa -5' |
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| 22144 | 3' | -57.9 | NC_004927.1 | + | 61805 | 0.71 | 0.375267 |
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Target: 5'- gCGCU-ACGAAgGCCCGCGAGUGGuucgGUg -3' miRNA: 3'- -GCGGcUGCUU-CGGGCGUUCGCCua--CA- -5' |
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| 22144 | 3' | -57.9 | NC_004927.1 | + | 56450 | 0.7 | 0.410082 |
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Target: 5'- aGCCgGAUGAAGCCCG---GUGGGUGg -3' miRNA: 3'- gCGG-CUGCUUCGGGCguuCGCCUACa -5' |
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| 22144 | 3' | -57.9 | NC_004927.1 | + | 35911 | 0.67 | 0.608587 |
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Target: 5'- aGCUGACac-GCCCGCAuccccucuuugAGCuGGAUGg -3' miRNA: 3'- gCGGCUGcuuCGGGCGU-----------UCG-CCUACa -5' |
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| 22144 | 3' | -57.9 | NC_004927.1 | + | 35823 | 0.84 | 0.051523 |
|
Target: 5'- gGUCGAUGAAGCCCGCAuuGUGGAUGa -3' miRNA: 3'- gCGGCUGCUUCGGGCGUu-CGCCUACa -5' |
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| 22144 | 3' | -57.9 | NC_004927.1 | + | 26942 | 0.66 | 0.640305 |
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Target: 5'- uCGCCcACGAGGUCgGCAAGCGu---- -3' miRNA: 3'- -GCGGcUGCUUCGGgCGUUCGCcuaca -5' |
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| 22144 | 3' | -57.9 | NC_004927.1 | + | 20688 | 0.68 | 0.566595 |
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Target: 5'- uCGCuCGAUGAAGUUaguaGCAAGUGGAg-- -3' miRNA: 3'- -GCG-GCUGCUUCGGg---CGUUCGCCUaca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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