miRNA display CGI


Results 1 - 9 of 9 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22144 3' -57.9 NC_004927.1 + 71110 0.69 0.505197
Target:  5'- -cCCGACGAGG---GCGAGCGGAUGg -3'
miRNA:   3'- gcGGCUGCUUCgggCGUUCGCCUACa -5'
22144 3' -57.9 NC_004927.1 + 68046 0.66 0.68664
Target:  5'- cCGCCGACGcaGauucggcaaacgcacAGCCCGCuguuGGCGuccuGAUGg -3'
miRNA:   3'- -GCGGCUGC--U---------------UCGGGCGu---UCGC----CUACa -5'
22144 3' -57.9 NC_004927.1 + 67932 0.69 0.479473
Target:  5'- gGCuCGAUGAAGCCuacgcaaggaacgcaCGCAGGCcggGGAUGa -3'
miRNA:   3'- gCG-GCUGCUUCGG---------------GCGUUCG---CCUACa -5'
22144 3' -57.9 NC_004927.1 + 61805 0.71 0.375267
Target:  5'- gCGCU-ACGAAgGCCCGCGAGUGGuucgGUg -3'
miRNA:   3'- -GCGGcUGCUU-CGGGCGUUCGCCua--CA- -5'
22144 3' -57.9 NC_004927.1 + 56450 0.7 0.410082
Target:  5'- aGCCgGAUGAAGCCCG---GUGGGUGg -3'
miRNA:   3'- gCGG-CUGCUUCGGGCguuCGCCUACa -5'
22144 3' -57.9 NC_004927.1 + 35911 0.67 0.608587
Target:  5'- aGCUGACac-GCCCGCAuccccucuuugAGCuGGAUGg -3'
miRNA:   3'- gCGGCUGcuuCGGGCGU-----------UCG-CCUACa -5'
22144 3' -57.9 NC_004927.1 + 35823 0.84 0.051523
Target:  5'- gGUCGAUGAAGCCCGCAuuGUGGAUGa -3'
miRNA:   3'- gCGGCUGCUUCGGGCGUu-CGCCUACa -5'
22144 3' -57.9 NC_004927.1 + 26942 0.66 0.640305
Target:  5'- uCGCCcACGAGGUCgGCAAGCGu---- -3'
miRNA:   3'- -GCGGcUGCUUCGGgCGUUCGCcuaca -5'
22144 3' -57.9 NC_004927.1 + 20688 0.68 0.566595
Target:  5'- uCGCuCGAUGAAGUUaguaGCAAGUGGAg-- -3'
miRNA:   3'- -GCG-GCUGCUUCGGg---CGUUCGCCUaca -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.