Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22145 | 5' | -54.6 | NC_004927.1 | + | 40539 | 0.66 | 0.849187 |
Target: 5'- uUCGGCCUCaacauGGUCGAAGacuaCCACGa -3' miRNA: 3'- -GGUUGGAGcuac-CCAGCUUCg---GGUGU- -5' |
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22145 | 5' | -54.6 | NC_004927.1 | + | 46678 | 0.66 | 0.857409 |
Target: 5'- uUCAACUUCGA-GGaGUCGAAccGCCaGCAc -3' miRNA: 3'- -GGUUGGAGCUaCC-CAGCUU--CGGgUGU- -5' |
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22145 | 5' | -54.6 | NC_004927.1 | + | 36475 | 0.68 | 0.743341 |
Target: 5'- aCAACCUCGAUGcguccguucgucacGGuUCGAccacGGCCCGa- -3' miRNA: 3'- gGUUGGAGCUAC--------------CC-AGCU----UCGGGUgu -5' |
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22145 | 5' | -54.6 | NC_004927.1 | + | 35837 | 0.69 | 0.673172 |
Target: 5'- cCCAcggaaGCCg----GGGUCGAugaAGCCCGCAu -3' miRNA: 3'- -GGU-----UGGagcuaCCCAGCU---UCGGGUGU- -5' |
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22145 | 5' | -54.6 | NC_004927.1 | + | 14578 | 0.69 | 0.704981 |
Target: 5'- aCGGCgCUCG-UGGGUCGccAGCCgACGu -3' miRNA: 3'- gGUUG-GAGCuACCCAGCu-UCGGgUGU- -5' |
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22145 | 5' | -54.6 | NC_004927.1 | + | 67950 | 0.7 | 0.598104 |
Target: 5'- aCGGCa-CGAUGGGUCGucggcucgaugAAGCCUACGc -3' miRNA: 3'- gGUUGgaGCUACCCAGC-----------UUCGGGUGU- -5' |
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22145 | 5' | -54.6 | NC_004927.1 | + | 32520 | 0.7 | 0.641029 |
Target: 5'- -uGACCgUCGA--GGUCGAugaAGCCCACGu -3' miRNA: 3'- ggUUGG-AGCUacCCAGCU---UCGGGUGU- -5' |
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22145 | 5' | -54.6 | NC_004927.1 | + | 68619 | 0.71 | 0.534689 |
Target: 5'- aCCGACCUCGAUGaGaUgGAGGCUgACGa -3' miRNA: 3'- -GGUUGGAGCUAC-CcAgCUUCGGgUGU- -5' |
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22145 | 5' | -54.6 | NC_004927.1 | + | 15337 | 0.73 | 0.425844 |
Target: 5'- cCCAAUcguaCUUGGUGaGGUCGggGCCgACGu -3' miRNA: 3'- -GGUUG----GAGCUAC-CCAGCuuCGGgUGU- -5' |
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22145 | 5' | -54.6 | NC_004927.1 | + | 69285 | 0.74 | 0.407483 |
Target: 5'- -uGACCUCGAUGGGUucaacgaguaCGggGUgCGCGa -3' miRNA: 3'- ggUUGGAGCUACCCA----------GCuuCGgGUGU- -5' |
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22145 | 5' | -54.6 | NC_004927.1 | + | 57573 | 0.76 | 0.308336 |
Target: 5'- uUCAACgUUGAUGGGUCGGuugacGGCCCGa- -3' miRNA: 3'- -GGUUGgAGCUACCCAGCU-----UCGGGUgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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