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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
22147 | 3' | -47.5 | NC_004927.1 | + | 71686 | 0.67 | 0.993594 |
Target: 5'- aAACgucauUAUGGAC-AUGACggGCGUGGg -3' miRNA: 3'- cUUGau---GUACCUGaUGCUGa-UGCACC- -5' |
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22147 | 3' | -47.5 | NC_004927.1 | + | 64291 | 0.71 | 0.923661 |
Target: 5'- cGACUACuaucucggaGUGGACUACGACgGCGa-- -3' miRNA: 3'- cUUGAUG---------UACCUGAUGCUGaUGCacc -5' |
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22147 | 3' | -47.5 | NC_004927.1 | + | 61459 | 0.73 | 0.882377 |
Target: 5'- cGACUcgGUGGACUAcgcgaccgcCGACUACGUGa -3' miRNA: 3'- cUUGAugUACCUGAU---------GCUGAUGCACc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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