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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22147 | 5' | -50.8 | NC_004927.1 | + | 961 | 0.67 | 0.939733 |
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Target: 5'- cCCCCUACaUUcCGUAUgggucaauuccgccCCGGUuccGUCUCCc -3' miRNA: 3'- -GGGGAUGcAA-GCAUA--------------GGUCG---UAGAGG- -5' |
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| 22147 | 5' | -50.8 | NC_004927.1 | + | 31563 | 0.74 | 0.633088 |
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Target: 5'- aCCCUGCGUacuuUCGgaaguagugcuggUCGGCGUCUCCu -3' miRNA: 3'- gGGGAUGCA----AGCaua----------GGUCGUAGAGG- -5' |
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| 22147 | 5' | -50.8 | NC_004927.1 | + | 34076 | 0.71 | 0.80005 |
|
Target: 5'- aCCaCCacggACGaUgGUuUCCAGCGUCUCCc -3' miRNA: 3'- -GG-GGa---UGCaAgCAuAGGUCGUAGAGG- -5' |
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| 22147 | 5' | -50.8 | NC_004927.1 | + | 34363 | 0.75 | 0.610099 |
|
Target: 5'- gCUCC-ACGUUCGUAUCCaaucacaucgAGCGugucuUCUCCg -3' miRNA: 3'- -GGGGaUGCAAGCAUAGG----------UCGU-----AGAGG- -5' |
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| 22147 | 5' | -50.8 | NC_004927.1 | + | 49168 | 0.66 | 0.961607 |
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Target: 5'- gCCCaCUACGaggacgauuggagagCGUccccuUCCGcGCAUCUCCg -3' miRNA: 3'- -GGG-GAUGCaa-------------GCAu----AGGU-CGUAGAGG- -5' |
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| 22147 | 5' | -50.8 | NC_004927.1 | + | 63264 | 0.68 | 0.919214 |
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Target: 5'- aCCacgaUGCGUUCGUggauAUUCGGCAgaacgUUCCg -3' miRNA: 3'- gGGg---AUGCAAGCA----UAGGUCGUa----GAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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