miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22148 3' -59.2 NC_004927.1 + 75453 0.66 0.624387
Target:  5'- uGUGuGGGGCCGUUguguACGGc-UAUGGUCg -3'
miRNA:   3'- gCGC-CCUCGGCAG----UGCCauGUGCCAG- -5'
22148 3' -59.2 NC_004927.1 + 31897 0.66 0.624387
Target:  5'- -uCGGGGGCUGUUACauccGCACGGUg -3'
miRNA:   3'- gcGCCCUCGGCAGUGcca-UGUGCCAg -5'
22148 3' -59.2 NC_004927.1 + 14603 0.67 0.572657
Target:  5'- uCGUGGGAGCgGUCugGagcGUucCACGG-Cg -3'
miRNA:   3'- -GCGCCCUCGgCAGugC---CAu-GUGCCaG- -5'
22148 3' -59.2 NC_004927.1 + 61736 0.67 0.54208
Target:  5'- cCGaGGGGGCCuaccCGGUcuACGCGGUCg -3'
miRNA:   3'- -GCgCCCUCGGcaguGCCA--UGUGCCAG- -5'
22148 3' -59.2 NC_004927.1 + 50394 0.69 0.417789
Target:  5'- uGcCGGGAGCgGUgGuggUGGUGCugGCGGUCa -3'
miRNA:   3'- gC-GCCCUCGgCAgU---GCCAUG--UGCCAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.