Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2215 | 3' | -53.2 | NC_001403.1 | + | 836 | 0.66 | 0.159606 |
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Target: 5'- gAUAGCaGGAUGUGCcaacgguuuuaggugUGGCCGuuucuCcGGCg -3' miRNA: 3'- -UGUCGcCCUAUACG---------------ACCGGUu----GuUCG- -5' |
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| 2215 | 3' | -53.2 | NC_001403.1 | + | 2404 | 0.66 | 0.142068 |
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Target: 5'- -aGGa-GGAgcUGUUGGCCAGCAGGa -3' miRNA: 3'- ugUCgcCCUauACGACCGGUUGUUCg -5' |
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| 2215 | 3' | -53.2 | NC_001403.1 | + | 1359 | 0.67 | 0.123703 |
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Target: 5'- uGCAGCGGGGaccugGC-GGCUcacuCAGGCu -3' miRNA: 3'- -UGUCGCCCUaua--CGaCCGGuu--GUUCG- -5' |
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| 2215 | 3' | -53.2 | NC_001403.1 | + | 2508 | 0.67 | 0.123703 |
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Target: 5'- -gAGCGGGugcacuUGCUGGgCAAgcGGCa -3' miRNA: 3'- ugUCGCCCuau---ACGACCgGUUguUCG- -5' |
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| 2215 | 3' | -53.2 | NC_001403.1 | + | 3943 | 0.67 | 0.118637 |
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Target: 5'- gGCGGCGGGGccggagagcgGCggGGCCGG-AGGCg -3' miRNA: 3'- -UGUCGCCCUaua-------CGa-CCGGUUgUUCG- -5' |
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| 2215 | 3' | -53.2 | NC_001403.1 | + | 3037 | 0.7 | 0.070241 |
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Target: 5'- aGCAGCGGGAUGguggGaaGGCCAuCGucuuccAGCc -3' miRNA: 3'- -UGUCGCCCUAUa---CgaCCGGUuGU------UCG- -5' |
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| 2215 | 3' | -53.2 | NC_001403.1 | + | 3457 | 0.7 | 0.067765 |
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Target: 5'- cGCGGCGGGcAUGgaguacCUGGaaAGCAAGCa -3' miRNA: 3'- -UGUCGCCC-UAUac----GACCggUUGUUCG- -5' |
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| 2215 | 3' | -53.2 | NC_001403.1 | + | 1746 | 0.83 | 0.006009 |
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Target: 5'- gGCAGCGGGGcc-GCUGGCCAAgcUGAGCa -3' miRNA: 3'- -UGUCGCCCUauaCGACCGGUU--GUUCG- -5' |
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| 2215 | 3' | -53.2 | NC_001403.1 | + | 2599 | 1.12 | 0.00002 |
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Target: 5'- cACAGCGGGAUAUGCUGGCCAACAAGCu -3' miRNA: 3'- -UGUCGCCCUAUACGACCGGUUGUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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