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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
22150 | 3' | -53 | NC_004927.1 | + | 22291 | 0.66 | 0.909661 |
Target: 5'- cGUCCGAcUUUCggacgUGCuucUCCCACCGcCc -3' miRNA: 3'- uCAGGCUaAAAG-----GCGu--AGGGUGGCuG- -5' |
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22150 | 3' | -53 | NC_004927.1 | + | 15265 | 0.66 | 0.896221 |
Target: 5'- --cCCGAggucUUCCGCAaugUCCuUGCCGACc -3' miRNA: 3'- ucaGGCUaa--AAGGCGU---AGG-GUGGCUG- -5' |
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22150 | 3' | -53 | NC_004927.1 | + | 18788 | 0.67 | 0.8412 |
Target: 5'- cGUCCaccggcUCCcaugcCAUCCCACCGACc -3' miRNA: 3'- uCAGGcuaaa-AGGc----GUAGGGUGGCUG- -5' |
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22150 | 3' | -53 | NC_004927.1 | + | 46354 | 0.68 | 0.794312 |
Target: 5'- cAGUCCGAc-UUCCcCAUCCCcgagauuACCGAg -3' miRNA: 3'- -UCAGGCUaaAAGGcGUAGGG-------UGGCUg -5' |
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22150 | 3' | -53 | NC_004927.1 | + | 2670 | 0.74 | 0.488352 |
Target: 5'- cGUUCGAU--UCCGcCGUCUCAUCGACu -3' miRNA: 3'- uCAGGCUAaaAGGC-GUAGGGUGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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