miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22154 3' -61.1 NC_004927.1 + 6403 0.66 0.535459
Target:  5'- --uUCCUcaCACCGGAGCuuugaGCCGCCa-- -3'
miRNA:   3'- cgcAGGAa-GUGGCCUCG-----CGGCGGccu -5'
22154 3' -61.1 NC_004927.1 + 21111 0.66 0.52555
Target:  5'- uGCGcUCCUcgaagUCGCCGcucGCGCCGCUGu- -3'
miRNA:   3'- -CGC-AGGA-----AGUGGCcu-CGCGGCGGCcu -5'
22154 3' -61.1 NC_004927.1 + 3373 0.66 0.52555
Target:  5'- uCGUUaugguaGCUGGAguaGCGCCGCCGGu -3'
miRNA:   3'- cGCAGgaag--UGGCCU---CGCGGCGGCCu -5'
22154 3' -61.1 NC_004927.1 + 34843 0.68 0.431068
Target:  5'- aGCGgCCaUCACgGGAaCGUCGUCGGGg -3'
miRNA:   3'- -CGCaGGaAGUGgCCUcGCGGCGGCCU- -5'
22154 3' -61.1 NC_004927.1 + 36432 0.7 0.315022
Target:  5'- aGCGagccauUCCUUCGCCGucucuaucgggucaGAGUGuuGCCGGu -3'
miRNA:   3'- -CGC------AGGAAGUGGC--------------CUCGCggCGGCCu -5'
22154 3' -61.1 NC_004927.1 + 36119 0.7 0.302904
Target:  5'- aGCGUCCccacUUCACCGGAaaCGCCGUCu-- -3'
miRNA:   3'- -CGCAGG----AAGUGGCCUc-GCGGCGGccu -5'
22154 3' -61.1 NC_004927.1 + 21826 0.71 0.268715
Target:  5'- uCGUCac-CGCCGGAGUcgccaccgagcgaGCCGCUGGAg -3'
miRNA:   3'- cGCAGgaaGUGGCCUCG-------------CGGCGGCCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.