Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22155 | 5' | -54.3 | NC_004927.1 | + | 11145 | 0.66 | 0.839804 |
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Target: 5'- cAGGGaggcgacuuggcuuUCGAGUCCGUgUCCcUCGUC-Ca -3' miRNA: 3'- -UCCC--------------AGCUUAGGUAgGGGaAGCAGaG- -5' |
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| 22155 | 5' | -54.3 | NC_004927.1 | + | 12209 | 0.66 | 0.824827 |
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Target: 5'- cGGGUUGu-UCCAaugCUCCgggggUUCGUCUCg -3' miRNA: 3'- uCCCAGCuuAGGUa--GGGG-----AAGCAGAG- -5' |
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| 22155 | 5' | -54.3 | NC_004927.1 | + | 42498 | 0.67 | 0.793219 |
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Target: 5'- cGGGUCGccggacuGAUuuuugugaccucgcCCGUCCCCUUCG-CUa -3' miRNA: 3'- uCCCAGC-------UUA--------------GGUAGGGGAAGCaGAg -5' |
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| 22155 | 5' | -54.3 | NC_004927.1 | + | 3239 | 0.67 | 0.77767 |
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Target: 5'- uGGGUCGGGauUCCAUCCCauauacUCGgccaaCUCc -3' miRNA: 3'- uCCCAGCUU--AGGUAGGGga----AGCa----GAG- -5' |
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| 22155 | 5' | -54.3 | NC_004927.1 | + | 945 | 0.68 | 0.737261 |
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Target: 5'- uGGGUC-AAUUCcgCCCCgguucCGUCUCc -3' miRNA: 3'- uCCCAGcUUAGGuaGGGGaa---GCAGAG- -5' |
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| 22155 | 5' | -54.3 | NC_004927.1 | + | 33958 | 0.76 | 0.29188 |
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Target: 5'- uGGcGUCGuAGUCCAUCCCCU-CGUCcCa -3' miRNA: 3'- uCC-CAGC-UUAGGUAGGGGAaGCAGaG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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