Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22156 | 3' | -55.6 | NC_004927.1 | + | 46473 | 0.69 | 0.611652 |
Target: 5'- cCAGCGGcgaccuuucGACuCGGGAG-GCCGUGGagugguacuUCGa -3' miRNA: 3'- -GUCGCC---------UUG-GCCCUCuCGGCAUU---------AGC- -5' |
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22156 | 3' | -55.6 | NC_004927.1 | + | 20323 | 0.69 | 0.633022 |
Target: 5'- gCGGCGGccccACCGGaGGGAGCguCGUcGUCGu -3' miRNA: 3'- -GUCGCCu---UGGCC-CUCUCG--GCAuUAGC- -5' |
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22156 | 3' | -55.6 | NC_004927.1 | + | 734 | 0.68 | 0.686268 |
Target: 5'- cCGGCGGAACCGGGccuuuuacGCCGgggAGUa- -3' miRNA: 3'- -GUCGCCUUGGCCCucu-----CGGCa--UUAgc -5' |
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22156 | 3' | -55.6 | NC_004927.1 | + | 423 | 0.68 | 0.661852 |
Target: 5'- aCGGaCGGAACCGGaGAGAcggacgggaaacggGCCGUAc--- -3' miRNA: 3'- -GUC-GCCUUGGCC-CUCU--------------CGGCAUuagc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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