miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22157 3' -53 NC_004927.1 + 4082 0.69 0.793916
Target:  5'- aGCUcGGuauguCGUGGUAGUCCAUuaucagaacCUCaGGa -3'
miRNA:   3'- gCGA-CCuu---GCACCAUCAGGUA---------GAG-CC- -5'
22157 3' -53 NC_004927.1 + 6148 0.68 0.847958
Target:  5'- aGCgUGGAAUcgguaGUGGUAGUcucugCCGUC-CGGg -3'
miRNA:   3'- gCG-ACCUUG-----CACCAUCA-----GGUAGaGCC- -5'
22157 3' -53 NC_004927.1 + 2926 0.67 0.887108
Target:  5'- uGCUGGAGaa-GGgcGUCCuUCUCGc -3'
miRNA:   3'- gCGACCUUgcaCCauCAGGuAGAGCc -5'
22157 3' -53 NC_004927.1 + 54023 0.66 0.894209
Target:  5'- cCGCUcucGGGGCGaGGUcaAGUUC-UCUCGGc -3'
miRNA:   3'- -GCGA---CCUUGCaCCA--UCAGGuAGAGCC- -5'
22157 3' -53 NC_004927.1 + 50332 0.66 0.907643
Target:  5'- cCGCUGGAggcggcggagcAgGUGGcAGUCUGcUCUCGa -3'
miRNA:   3'- -GCGACCU-----------UgCACCaUCAGGU-AGAGCc -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.