miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22158 5' -50.3 NC_004927.1 + 23601 0.74 0.643338
Target:  5'- gCCUgCcGAgCGGU-CUGCUUCGUGUCCu -3'
miRNA:   3'- -GGA-GaCUgGUUAuGACGAAGCGCAGG- -5'
22158 5' -50.3 NC_004927.1 + 8364 0.71 0.801546
Target:  5'- aCCUCccaccGAcucCCAAUACUGCUcgaUGUGUCCg -3'
miRNA:   3'- -GGAGa----CU---GGUUAUGACGAa--GCGCAGG- -5'
22158 5' -50.3 NC_004927.1 + 58526 0.7 0.855762
Target:  5'- aCCUCgaugcucgcuaUGGCCGGUGCUGgUgcgGCGUUCa -3'
miRNA:   3'- -GGAG-----------ACUGGUUAUGACgAag-CGCAGG- -5'
22158 5' -50.3 NC_004927.1 + 1796 0.66 0.970668
Target:  5'- cCCUUUaGGCCG--ACUGCUauaCGCG-CCg -3'
miRNA:   3'- -GGAGA-CUGGUuaUGACGAa--GCGCaGG- -5'
22158 5' -50.3 NC_004927.1 + 26049 0.66 0.976421
Target:  5'- gUUCUGACC-GUGCcggauguggGCcUUGUGUCCa -3'
miRNA:   3'- gGAGACUGGuUAUGa--------CGaAGCGCAGG- -5'
22158 5' -50.3 NC_004927.1 + 22092 0.66 0.978965
Target:  5'- aCCgacCUGuACCucaguaacGUAUUGCUUgGCGUCUu -3'
miRNA:   3'- -GGa--GAC-UGGu-------UAUGACGAAgCGCAGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.