miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22159 5' -49.9 NC_004927.1 + 55365 0.7 0.831389
Target:  5'- cGGACAcauCCAGUcuaucACGGAGGG-UUCUCg -3'
miRNA:   3'- aCCUGU---GGUCAu----UGCCUCUUgAAGAG- -5'
22159 5' -49.9 NC_004927.1 + 32097 0.69 0.889666
Target:  5'- gGGACGUagaAGUAgGCGGGGAAgUUCUCc -3'
miRNA:   3'- aCCUGUGg--UCAU-UGCCUCUUgAAGAG- -5'
22159 5' -49.9 NC_004927.1 + 8605 0.68 0.934738
Target:  5'- cGaGGCuCCGGUAGCguucguggguguGGAGAGCUUCg- -3'
miRNA:   3'- aC-CUGuGGUCAUUG------------CCUCUUGAAGag -5'
22159 5' -49.9 NC_004927.1 + 50288 0.67 0.940032
Target:  5'- gGGAgUGCCGGUAACGGAGuGACa---- -3'
miRNA:   3'- aCCU-GUGGUCAUUGCCUC-UUGaagag -5'
22159 5' -49.9 NC_004927.1 + 60990 0.67 0.943568
Target:  5'- gUGaGugACCAG-AACGGAGAugaggcggguauccGCUUCg- -3'
miRNA:   3'- -AC-CugUGGUCaUUGCCUCU--------------UGAAGag -5'
22159 5' -49.9 NC_004927.1 + 30003 0.66 0.975367
Target:  5'- cGGA-AUCAGgcagGACGGAGGaacGCUUcCUCa -3'
miRNA:   3'- aCCUgUGGUCa---UUGCCUCU---UGAA-GAG- -5'
22159 5' -49.9 NC_004927.1 + 55325 0.66 0.975367
Target:  5'- aUGGACAa----GACGGAGGACaaUCUCa -3'
miRNA:   3'- -ACCUGUggucaUUGCCUCUUGa-AGAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.