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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22160 | 5' | -50.6 | NC_004927.1 | + | 31490 | 0.71 | 0.791628 |
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Target: 5'- --cCGAccucGGUUGcUAUCCUCUCCGAa- -3' miRNA: 3'- auaGCU----CCAGCaAUAGGAGAGGCUac -5' |
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| 22160 | 5' | -50.6 | NC_004927.1 | + | 29534 | 0.7 | 0.829731 |
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Target: 5'- uUGUCGuAGGUCGau-UCCaUCUCgGAUGa -3' miRNA: 3'- -AUAGC-UCCAGCaauAGG-AGAGgCUAC- -5' |
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| 22160 | 5' | -50.6 | NC_004927.1 | + | 3809 | 0.69 | 0.856111 |
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Target: 5'- cAUCGA-GUCgGUUGUCCcCUCCGAg- -3' miRNA: 3'- aUAGCUcCAG-CAAUAGGaGAGGCUac -5' |
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| 22160 | 5' | -50.6 | NC_004927.1 | + | 38869 | 0.69 | 0.871718 |
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Target: 5'- aGUCGGGGUgaUGUguUCCUCgauuagcUCCGGUGg -3' miRNA: 3'- aUAGCUCCA--GCAauAGGAG-------AGGCUAC- -5' |
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| 22160 | 5' | -50.6 | NC_004927.1 | + | 9708 | 0.67 | 0.943518 |
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Target: 5'- cGUCcGGGUCGUacAUCCgccaCUCCGAUu -3' miRNA: 3'- aUAGcUCCAGCAa-UAGGa---GAGGCUAc -5' |
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| 22160 | 5' | -50.6 | NC_004927.1 | + | 75384 | 0.66 | 0.948294 |
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Target: 5'- cAUCGAGGguggUGUCCUCaUCUGAc- -3' miRNA: 3'- aUAGCUCCagcaAUAGGAG-AGGCUac -5' |
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| 22160 | 5' | -50.6 | NC_004927.1 | + | 15435 | 0.66 | 0.957023 |
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Target: 5'- -cUCGGGGUCGUcGU--UCUCCGGg- -3' miRNA: 3'- auAGCUCCAGCAaUAggAGAGGCUac -5' |
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| 22160 | 5' | -50.6 | NC_004927.1 | + | 42803 | 0.66 | 0.960602 |
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Target: 5'- aAUCGGGGUCGUcgUuguaucgcuucacGUCCUCgauguaCCGAUu -3' miRNA: 3'- aUAGCUCCAGCA--A-------------UAGGAGa-----GGCUAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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