miRNA display CGI


Results 1 - 8 of 8 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22164 3' -52.3 NC_004927.1 + 7066 0.65 0.923524
Target:  5'- cGAAGgacuugagccacaCCGAGAGAcAGCu-UCGCAGAg -3'
miRNA:   3'- uCUUCa------------GGCUCUCU-UCGuuGGCGUCU- -5'
22164 3' -52.3 NC_004927.1 + 12566 0.69 0.794095
Target:  5'- uAGAGGUcgccgucccaaagcgCCGAGaAGAAGUGACCGCc-- -3'
miRNA:   3'- -UCUUCA---------------GGCUC-UCUUCGUUGGCGucu -5'
22164 3' -52.3 NC_004927.1 + 20552 0.67 0.886141
Target:  5'- gAGAAGUgaGAGAGuccAGCAGa-GCAGAa -3'
miRNA:   3'- -UCUUCAggCUCUCu--UCGUUggCGUCU- -5'
22164 3' -52.3 NC_004927.1 + 23807 0.67 0.862698
Target:  5'- cAGAGcuGUCCGAGucgucGGAGuCAccACCGUAGAg -3'
miRNA:   3'- -UCUU--CAGGCUCu----CUUC-GU--UGGCGUCU- -5'
22164 3' -52.3 NC_004927.1 + 26565 0.66 0.919931
Target:  5'- ---cGUCCGGGGu-GGUAGCgGCGGAg -3'
miRNA:   3'- ucuuCAGGCUCUcuUCGUUGgCGUCU- -5'
22164 3' -52.3 NC_004927.1 + 30690 0.67 0.886141
Target:  5'- cGGGAGUCCGAcucggaaccGAGAGGgAAC-GCGGu -3'
miRNA:   3'- -UCUUCAGGCU---------CUCUUCgUUGgCGUCu -5'
22164 3' -52.3 NC_004927.1 + 32771 0.67 0.87858
Target:  5'- --cGGUgCGAGAGAGGCGgucggcguugacACCGCGc- -3'
miRNA:   3'- ucuUCAgGCUCUCUUCGU------------UGGCGUcu -5'
22164 3' -52.3 NC_004927.1 + 42190 0.68 0.809507
Target:  5'- uAGGAG-CCGAgcGAGAAGCGcgugGCCGCu-- -3'
miRNA:   3'- -UCUUCaGGCU--CUCUUCGU----UGGCGucu -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.