miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22165 3' -51 NC_004927.1 + 11678 0.7 0.831013
Target:  5'- uCUUCGCCGUCcccacUCaucgagccgACGAUGGUgUUCGu -3'
miRNA:   3'- -GAAGCGGUAGc----AG---------UGCUACCAgAAGC- -5'
22165 3' -51 NC_004927.1 + 56849 0.67 0.932839
Target:  5'- aUUCGUCcUC-UCACGAUGGaCUUCc -3'
miRNA:   3'- gAAGCGGuAGcAGUGCUACCaGAAGc -5'
22165 3' -51 NC_004927.1 + 25412 0.66 0.952323
Target:  5'- gCUUCGUCuUCGUCGCuuucGUGGUCg--- -3'
miRNA:   3'- -GAAGCGGuAGCAGUGc---UACCAGaagc -5'
22165 3' -51 NC_004927.1 + 4569 0.66 0.960466
Target:  5'- gCUUCGCUuagGUCGaUACGccGGUCgUCGg -3'
miRNA:   3'- -GAAGCGG---UAGCaGUGCuaCCAGaAGC- -5'
22165 3' -51 NC_004927.1 + 46807 0.66 0.963073
Target:  5'- --aCGCUccgcgagaagugggAggGUCACGAgGGUCUUCGg -3'
miRNA:   3'- gaaGCGG--------------UagCAGUGCUaCCAGAAGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.