miRNA: 3'- -AGGCUACUCg---CaCUUGUACU---GCUauu -5'
miRNA: 3'- aGGCU------ACUCGCACUUGua---CUGCUauu -5'
miRNA: 3'- aGGCUACUCGcACUUGUa--CUGCUauu -5'
miRNA: 3'- -AGGC---UACuCG-CACUugUACUGCUAUu -5'
miRNA: 3'- aGGCUACUCGCaCUUGUac-UGCUAUu -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22165 | 5' | -48.8 | NC_004927.1 | + | 10137 | 0.66 | 0.984441 |
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Target: 5'- uUCCGGUGAGaguaGaGAACAUGAacuCGAa-- -3' miRNA: 3'- -AGGCUACUCg---CaCUUGUACU---GCUauu -5' |
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| 22165 | 5' | -48.8 | NC_004927.1 | + | 46846 | 0.67 | 0.974765 |
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Target: 5'- gCCGAcgaaauUGAGUGUGAGCuguacGGCGAg-- -3' miRNA: 3'- aGGCU------ACUCGCACUUGua---CUGCUauu -5' |
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| 22165 | 5' | -48.8 | NC_004927.1 | + | 47469 | 0.68 | 0.957287 |
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Target: 5'- gCCGAUGAGUuUGAACAccaGAUGAa-- -3' miRNA: 3'- aGGCUACUCGcACUUGUa--CUGCUauu -5' |
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| 22165 | 5' | -48.8 | NC_004927.1 | + | 50760 | 0.68 | 0.943547 |
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Target: 5'- cUCCGcacAUG-GCgGUGAugGUGGCGAUGc -3' miRNA: 3'- -AGGC---UACuCG-CACUugUACUGCUAUu -5' |
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| 22165 | 5' | -48.8 | NC_004927.1 | + | 61633 | 0.7 | 0.90131 |
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Target: 5'- gCCGGUGAGCGaGGACGcaaACGAUGu -3' miRNA: 3'- aGGCUACUCGCaCUUGUac-UGCUAUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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