miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22166 3' -53.3 NC_004927.1 + 134 0.66 0.871585
Target:  5'- -gGGACAGgGGACG-----GGGGCCGg -3'
miRNA:   3'- gaCUUGUCgCUUGCguuagUCCCGGC- -5'
22166 3' -53.3 NC_004927.1 + 75651 0.66 0.871585
Target:  5'- -gGGACAGgGGACG-----GGGGCCGg -3'
miRNA:   3'- gaCUUGUCgCUUGCguuagUCCCGGC- -5'
22166 3' -53.3 NC_004927.1 + 26703 0.66 0.855419
Target:  5'- -cGAGCGGCuacuuCGCAGUCcGGGCa- -3'
miRNA:   3'- gaCUUGUCGcuu--GCGUUAGuCCCGgc -5'
22166 3' -53.3 NC_004927.1 + 34845 0.66 0.855419
Target:  5'- -cGAGCGGCcaucacggGAACGUcGUCGGGGUUc -3'
miRNA:   3'- gaCUUGUCG--------CUUGCGuUAGUCCCGGc -5'
22166 3' -53.3 NC_004927.1 + 20370 0.67 0.846985
Target:  5'- uUGG--AGCGAGCGguAcuUCcGGGCCGg -3'
miRNA:   3'- gACUugUCGCUUGCguU--AGuCCCGGC- -5'
22166 3' -53.3 NC_004927.1 + 30676 0.67 0.838329
Target:  5'- -gGAACcgagAGgGAACGCGGUC-GGGCUu -3'
miRNA:   3'- gaCUUG----UCgCUUGCGUUAGuCCCGGc -5'
22166 3' -53.3 NC_004927.1 + 17498 0.69 0.70995
Target:  5'- -cGAACAGCccGAACacgGCAGUCugAGGGUCGc -3'
miRNA:   3'- gaCUUGUCG--CUUG---CGUUAG--UCCCGGC- -5'
22166 3' -53.3 NC_004927.1 + 59583 0.7 0.688452
Target:  5'- -gGGGCcGCGAGCGUAAUCuucGGCCu -3'
miRNA:   3'- gaCUUGuCGCUUGCGUUAGuc-CCGGc -5'
22166 3' -53.3 NC_004927.1 + 14224 0.71 0.601156
Target:  5'- aUGAACGuCGuguCGCAAUCGGGGCa- -3'
miRNA:   3'- gACUUGUcGCuu-GCGUUAGUCCCGgc -5'
22166 3' -53.3 NC_004927.1 + 42876 0.73 0.474392
Target:  5'- -aGGACAGCGAACGgGugaauGUCGaGGCCGa -3'
miRNA:   3'- gaCUUGUCGCUUGCgU-----UAGUcCCGGC- -5'
22166 3' -53.3 NC_004927.1 + 53850 0.79 0.231606
Target:  5'- -cGAACccggAGCGAGCGCAaacGUCGGGGCUa -3'
miRNA:   3'- gaCUUG----UCGCUUGCGU---UAGUCCCGGc -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.