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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22168 | 5' | -60.2 | NC_004927.1 | + | 46341 | 0.75 | 0.164903 |
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Target: 5'- cCAUCCCCGaGauuaCCGAGCGCGucGGUGg -3' miRNA: 3'- aGUAGGGGCgCg---GGCUUGCGCu-CCAC- -5' |
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| 22168 | 5' | -60.2 | NC_004927.1 | + | 58977 | 0.74 | 0.178065 |
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Target: 5'- cCA-CCUCGCGUUCGGGCGCGGGGaUGa -3' miRNA: 3'- aGUaGGGGCGCGGGCUUGCGCUCC-AC- -5' |
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| 22168 | 5' | -60.2 | NC_004927.1 | + | 62632 | 0.69 | 0.37468 |
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Target: 5'- uUCAUgcaCCCCG-GCCCGAACG-GGGGc- -3' miRNA: 3'- -AGUA---GGGGCgCGGGCUUGCgCUCCac -5' |
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| 22168 | 5' | -60.2 | NC_004927.1 | + | 73650 | 0.67 | 0.491895 |
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Target: 5'- --uUCCCCgGCGUaaCCGGGgcCGCGAGGUc -3' miRNA: 3'- aguAGGGG-CGCG--GGCUU--GCGCUCCAc -5' |
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| 22168 | 5' | -60.2 | NC_004927.1 | + | 51348 | 0.66 | 0.531304 |
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Target: 5'- --uUCCCCGa-CCCGGACGaGAGGa- -3' miRNA: 3'- aguAGGGGCgcGGGCUUGCgCUCCac -5' |
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| 22168 | 5' | -60.2 | NC_004927.1 | + | 24129 | 0.66 | 0.561594 |
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Target: 5'- gUUGUCCCCGuCGUCCGuGugGCcccGAGGg- -3' miRNA: 3'- -AGUAGGGGC-GCGGGC-UugCG---CUCCac -5' |
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| 22168 | 5' | -60.2 | NC_004927.1 | + | 40616 | 0.66 | 0.582049 |
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Target: 5'- aUAUaccaCCCGCGCCCGAACccauCGAGu-- -3' miRNA: 3'- aGUAg---GGGCGCGGGCUUGc---GCUCcac -5' |
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| 22168 | 5' | -60.2 | NC_004927.1 | + | 41183 | 0.66 | 0.586159 |
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Target: 5'- gUCGUCCUugauuuguuccaucuCGUGUUCGAggaAgGCGAGGUGu -3' miRNA: 3'- -AGUAGGG---------------GCGCGGGCU---UgCGCUCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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