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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 22170 | 5' | -57.1 | NC_004927.1 | + | 73972 | 0.66 | 0.660581 |
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Target: 5'- gAGAAGACCg-CGAGCGCgacggaacagcuCGC-CGCAg -3' miRNA: 3'- -UCUUCUGGgaGUUCGCG------------GCGuGCGUg -5' |
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| 22170 | 5' | -57.1 | NC_004927.1 | + | 67371 | 0.67 | 0.585476 |
|
Target: 5'- gGGAAGGCCCUCG---GCUuCACGCAg -3' miRNA: 3'- -UCUUCUGGGAGUucgCGGcGUGCGUg -5' |
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| 22170 | 5' | -57.1 | NC_004927.1 | + | 61561 | 0.68 | 0.543182 |
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Target: 5'- cGAAGACCCgagCGAGCugacucccGgCGCugGCGa -3' miRNA: 3'- uCUUCUGGGa--GUUCG--------CgGCGugCGUg -5' |
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| 22170 | 5' | -57.1 | NC_004927.1 | + | 55918 | 0.68 | 0.553674 |
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Target: 5'- cGAAGA-CCUCGAuGCGuCUGCcgACGCGCu -3' miRNA: 3'- uCUUCUgGGAGUU-CGC-GGCG--UGCGUG- -5' |
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| 22170 | 5' | -57.1 | NC_004927.1 | + | 75123 | 0.68 | 0.564225 |
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Target: 5'- cGAAcccGGCCCUCGcGUGUCGUGCGCu- -3' miRNA: 3'- uCUU---CUGGGAGUuCGCGGCGUGCGug -5' |
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| 22170 | 5' | -57.1 | NC_004927.1 | + | 21118 | 0.71 | 0.379183 |
|
Target: 5'- aGGGAGAugcgcUCCUCgAAGuCGCCGCuCGCGCc -3' miRNA: 3'- -UCUUCU-----GGGAG-UUC-GCGGCGuGCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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