Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
22174 | 3' | -49.6 | NC_004927.1 | + | 69129 | 0.66 | 0.9756 |
Target: 5'- -uGAGAAGaAUUAC-UCGGGUCAGg- -3' miRNA: 3'- cuCUCUUCcUGAUGuAGCUCAGUCau -5' |
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22174 | 3' | -49.6 | NC_004927.1 | + | 20860 | 0.66 | 0.974751 |
Target: 5'- cGAGGGAAGGACUcgaACcuUCGAcuaccugauuaacaGUCAGg- -3' miRNA: 3'- -CUCUCUUCCUGA---UGu-AGCU--------------CAGUCau -5' |
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22174 | 3' | -49.6 | NC_004927.1 | + | 11145 | 0.68 | 0.929024 |
Target: 5'- cAGGGAGGcGACUugGcuuUCGAGUCcGUGu -3' miRNA: 3'- cUCUCUUC-CUGAugU---AGCUCAGuCAU- -5' |
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22174 | 3' | -49.6 | NC_004927.1 | + | 7032 | 0.68 | 0.916863 |
Target: 5'- cAGAGAAGGACUGuCAUauagcuaccagCGAGUCcuccGGUGu -3' miRNA: 3'- cUCUCUUCCUGAU-GUA-----------GCUCAG----UCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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