Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22174 | 5' | -53.5 | NC_004927.1 | + | 31731 | 0.87 | 0.071664 |
Target: 5'- -cCUCCCCCUCGAUGAUgguguUCGCUUCa- -3' miRNA: 3'- uaGAGGGGGAGCUACUA-----AGCGAAGcg -5' |
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22174 | 5' | -53.5 | NC_004927.1 | + | 43921 | 0.77 | 0.301683 |
Target: 5'- cUCgaaCCCCCUCGAUGAUUU-CUUCGUc -3' miRNA: 3'- uAGa--GGGGGAGCUACUAAGcGAAGCG- -5' |
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22174 | 5' | -53.5 | NC_004927.1 | + | 5327 | 0.71 | 0.612743 |
Target: 5'- -gCUCaUCCCUUGAUGAUuuggaguccacgguUCGCUUCGa -3' miRNA: 3'- uaGAG-GGGGAGCUACUA--------------AGCGAAGCg -5' |
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22174 | 5' | -53.5 | NC_004927.1 | + | 25321 | 0.69 | 0.702983 |
Target: 5'- uUCgaccuugCCCCCgUCGAUGcgUCuuUUCGCa -3' miRNA: 3'- uAGa------GGGGG-AGCUACuaAGcgAAGCG- -5' |
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22174 | 5' | -53.5 | NC_004927.1 | + | 15211 | 0.69 | 0.717908 |
Target: 5'- cUCaUCCCCCUCGAUucuuucgaccgccgcGAgccaCGCUUgGCg -3' miRNA: 3'- uAG-AGGGGGAGCUA---------------CUaa--GCGAAgCG- -5' |
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22174 | 5' | -53.5 | NC_004927.1 | + | 27639 | 0.69 | 0.724256 |
Target: 5'- aGUUUCagaacgCCCCUCGAUGAUUuccugCGCUUCu- -3' miRNA: 3'- -UAGAG------GGGGAGCUACUAA-----GCGAAGcg -5' |
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22174 | 5' | -53.5 | NC_004927.1 | + | 68443 | 0.68 | 0.785548 |
Target: 5'- aGUCUCgCCUCUCGGUGAggacgaGCUg-GCa -3' miRNA: 3'- -UAGAG-GGGGAGCUACUaag---CGAagCG- -5' |
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22174 | 5' | -53.5 | NC_004927.1 | + | 34114 | 0.68 | 0.795277 |
Target: 5'- -gCUCCCUCUCGgAUGAUgcggCGaaugucgUCGCu -3' miRNA: 3'- uaGAGGGGGAGC-UACUAa---GCga-----AGCG- -5' |
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22174 | 5' | -53.5 | NC_004927.1 | + | 30437 | 0.67 | 0.804839 |
Target: 5'- --aUCUCCCUCGGUGGUcggGCUuUCGUa -3' miRNA: 3'- uagAGGGGGAGCUACUAag-CGA-AGCG- -5' |
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22174 | 5' | -53.5 | NC_004927.1 | + | 5058 | 0.67 | 0.808614 |
Target: 5'- -cCUCCguCCCUCGGUcgaucuccagaacagGAUUCGCgUUGCu -3' miRNA: 3'- uaGAGG--GGGAGCUA---------------CUAAGCGaAGCG- -5' |
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22174 | 5' | -53.5 | NC_004927.1 | + | 42439 | 0.67 | 0.820678 |
Target: 5'- -gCUUgCCCUCGAUGcgugggaacgggucAUUUGCUcCGCg -3' miRNA: 3'- uaGAGgGGGAGCUAC--------------UAAGCGAaGCG- -5' |
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22174 | 5' | -53.5 | NC_004927.1 | + | 73171 | 0.67 | 0.8412 |
Target: 5'- cUCUCCCCCUCGAUGcggUGaaUCu- -3' miRNA: 3'- uAGAGGGGGAGCUACuaaGCgaAGcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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