miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22177 3' -59.2 NC_004927.1 + 36020 0.71 0.310727
Target:  5'- gGAAGUCCUCGUagaGCCGGGaaggAGUCGc -3'
miRNA:   3'- -CUUCAGGAGCGgggCGGCCU----UCAGCa -5'
22177 3' -59.2 NC_004927.1 + 21838 0.69 0.4347
Target:  5'- gGAAGccCCUCGUCgucaCCGCCGG-AGUCGc -3'
miRNA:   3'- -CUUCa-GGAGCGG----GGCGGCCuUCAGCa -5'
22177 3' -59.2 NC_004927.1 + 37865 0.68 0.462737
Target:  5'- ---cUCCUUGUucaCCGCaCGGAAGUCGUc -3'
miRNA:   3'- cuucAGGAGCGg--GGCG-GCCUUCAGCA- -5'
22177 3' -59.2 NC_004927.1 + 43771 0.68 0.472292
Target:  5'- --cGUCCUCGaagacUCCUGaaucaCGGAAGUCGUa -3'
miRNA:   3'- cuuCAGGAGC-----GGGGCg----GCCUUCAGCA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.