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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22178 | 3' | -50.3 | NC_004927.1 | + | 36564 | 0.66 | 0.978965 |
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Target: 5'- cCGUCcgGUagaAGUCCGuCUCCu-GAUAGUGGa -3' miRNA: 3'- -GCAG--CA---UCAGGU-GAGGcuCUAUCAUC- -5' |
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| 22178 | 3' | -50.3 | NC_004927.1 | + | 29478 | 0.66 | 0.97337 |
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Target: 5'- aGUCGgcGUCCACUuuGucgucGAUuucguccGGUGGg -3' miRNA: 3'- gCAGCauCAGGUGAggCu----CUA-------UCAUC- -5' |
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| 22178 | 3' | -50.3 | NC_004927.1 | + | 12013 | 0.66 | 0.970668 |
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Target: 5'- aGUCGU--UCCGCUCauCGAGcaGGUAGg -3' miRNA: 3'- gCAGCAucAGGUGAG--GCUCuaUCAUC- -5' |
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| 22178 | 3' | -50.3 | NC_004927.1 | + | 42218 | 0.66 | 0.967442 |
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Target: 5'- aGUCGUGGUCUuccgucGCUuuG-GcgAGUAGg -3' miRNA: 3'- gCAGCAUCAGG------UGAggCuCuaUCAUC- -5' |
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| 22178 | 3' | -50.3 | NC_004927.1 | + | 3804 | 0.68 | 0.937699 |
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Target: 5'- aGUCGguuGUCCcCUCCGAGAa----- -3' miRNA: 3'- gCAGCau-CAGGuGAGGCUCUaucauc -5' |
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| 22178 | 3' | -50.3 | NC_004927.1 | + | 37994 | 0.68 | 0.914716 |
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Target: 5'- aGUCGaAGUCCAUcgcgUCGAGA-AGUGGa -3' miRNA: 3'- gCAGCaUCAGGUGa---GGCUCUaUCAUC- -5' |
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| 22178 | 3' | -50.3 | NC_004927.1 | + | 26604 | 0.71 | 0.816722 |
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Target: 5'- uCGUCGUAGUagcccuccaucagcUCGCcgucaaucauccgUCCGGGGUGGUAGc -3' miRNA: 3'- -GCAGCAUCA--------------GGUG-------------AGGCUCUAUCAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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