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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22179 | 3' | -52.9 | NC_004927.1 | + | 2262 | 0.72 | 0.598203 |
|
Target: 5'- uGUGAA-----CCUCGCGGGGCGCUUCg -3' miRNA: 3'- -CACUUccauaGGAGCGCCUUGUGGAG- -5' |
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| 22179 | 3' | -52.9 | NC_004927.1 | + | 11638 | 0.67 | 0.854223 |
|
Target: 5'- uGUGAAucagccGGUGuUCCUCGgGGAACGgCaUCg -3' miRNA: 3'- -CACUU------CCAU-AGGAGCgCCUUGUgG-AG- -5' |
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| 22179 | 3' | -52.9 | NC_004927.1 | + | 18303 | 0.67 | 0.8625 |
|
Target: 5'- -cGAAGG-AUuaUCUCGUGGGauGCACCUUc -3' miRNA: 3'- caCUUCCaUA--GGAGCGCCU--UGUGGAG- -5' |
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| 22179 | 3' | -52.9 | NC_004927.1 | + | 27664 | 0.71 | 0.642159 |
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Target: 5'- -gGAAGGaAUCUUCG-GGAGCGCCa- -3' miRNA: 3'- caCUUCCaUAGGAGCgCCUUGUGGag -5' |
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| 22179 | 3' | -52.9 | NC_004927.1 | + | 64402 | 0.67 | 0.828031 |
|
Target: 5'- --cGAGGUGUUC-CGCGaGGAUACCUUc -3' miRNA: 3'- cacUUCCAUAGGaGCGC-CUUGUGGAG- -5' |
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| 22179 | 3' | -52.9 | NC_004927.1 | + | 68713 | 0.67 | 0.854223 |
|
Target: 5'- uGUGggGGaGUCCUCG-GaGAACucgGCCUUc -3' miRNA: 3'- -CACuuCCaUAGGAGCgC-CUUG---UGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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