Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2218 | 3' | -64.1 | NC_001405.1 | + | 9780 | 0.66 | 0.221219 |
Target: 5'- cCGCUGCCGcCCGCCacgGuGCUcagCCUaCCUu -3' miRNA: 3'- -GCGGCGGC-GGCGGa--C-CGAa--GGA-GGAc -5' |
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2218 | 3' | -64.1 | NC_001405.1 | + | 17514 | 0.66 | 0.217823 |
Target: 5'- cCGCCGCCGUCGCCgucgccagcccgugcUGGCc-CCgauuUCCg- -3' miRNA: 3'- -GCGGCGGCGGCGG---------------ACCGaaGG----AGGac -5' |
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2218 | 3' | -64.1 | NC_001405.1 | + | 10132 | 0.66 | 0.215585 |
Target: 5'- gCGuuGUCGCCGaCCUGGC--CCUgCUu -3' miRNA: 3'- -GCggCGGCGGC-GGACCGaaGGAgGAc -5' |
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2218 | 3' | -64.1 | NC_001405.1 | + | 18795 | 0.66 | 0.204685 |
Target: 5'- aGCCGCCGUgCGCCcgcuuuccaagaUGGCUaCC-CCUu -3' miRNA: 3'- gCGGCGGCG-GCGG------------ACCGAaGGaGGAc -5' |
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2218 | 3' | -64.1 | NC_001405.1 | + | 10381 | 0.66 | 0.194267 |
Target: 5'- aGCCGCCGCCGCacuuuuUGGUgggauaUCaguaCCUGg -3' miRNA: 3'- gCGGCGGCGGCGg-----ACCGa-----AGga--GGAC- -5' |
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2218 | 3' | -64.1 | NC_001405.1 | + | 16386 | 0.67 | 0.184315 |
Target: 5'- gGCCGCgGCUGCUgcGGCggCCgcucgucgCCUGg -3' miRNA: 3'- gCGGCGgCGGCGGa-CCGaaGGa-------GGAC- -5' |
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2218 | 3' | -64.1 | NC_001405.1 | + | 23635 | 0.67 | 0.17951 |
Target: 5'- aGCCGCCGCa-CCgUGcGCUUaCCUCCc- -3' miRNA: 3'- gCGGCGGCGgcGG-AC-CGAA-GGAGGac -5' |
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2218 | 3' | -64.1 | NC_001405.1 | + | 26878 | 0.67 | 0.164874 |
Target: 5'- uGCUGCCGCCGCUguggauuucuugGGCUUUgUCa-- -3' miRNA: 3'- gCGGCGGCGGCGGa-----------CCGAAGgAGgac -5' |
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2218 | 3' | -64.1 | NC_001405.1 | + | 11198 | 0.68 | 0.161386 |
Target: 5'- uGCCGCCGCgucaGCCgcggauGUUgccCCUCCUGa -3' miRNA: 3'- gCGGCGGCGg---CGGac----CGAa--GGAGGAC- -5' |
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2218 | 3' | -64.1 | NC_001405.1 | + | 16260 | 0.68 | 0.161386 |
Target: 5'- gGCCGCCGCCGCgCguugGGCggCagugCCg- -3' miRNA: 3'- gCGGCGGCGGCG-Ga---CCGaaGga--GGac -5' |
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2218 | 3' | -64.1 | NC_001405.1 | + | 15247 | 0.68 | 0.144928 |
Target: 5'- uGCCGCUGCCucgGCCUcagcGcGCUucUCCUCCg- -3' miRNA: 3'- gCGGCGGCGG---CGGA----C-CGA--AGGAGGac -5' |
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2218 | 3' | -64.1 | NC_001405.1 | + | 23994 | 0.69 | 0.130015 |
Target: 5'- uCGCCGUCGCCGCCg-----CCUCCc- -3' miRNA: 3'- -GCGGCGGCGGCGGaccgaaGGAGGac -5' |
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2218 | 3' | -64.1 | NC_001405.1 | + | 15104 | 0.72 | 0.076783 |
Target: 5'- uGuuGCCGCCGCCUGcGCcaUCCccgcCCUGu -3' miRNA: 3'- gCggCGGCGGCGGAC-CGa-AGGa---GGAC- -5' |
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2218 | 3' | -64.1 | NC_001405.1 | + | 33230 | 0.72 | 0.068602 |
Target: 5'- uGCUGCCGCCGCC--GCU-CCgUCCUGc -3' miRNA: 3'- gCGGCGGCGGCGGacCGAaGG-AGGAC- -5' |
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2218 | 3' | -64.1 | NC_001405.1 | + | 2220 | 1.07 | 0.000114 |
Target: 5'- cCGCCGCCGCCGCCUGGCUUCCUCCUGc -3' miRNA: 3'- -GCGGCGGCGGCGGACCGAAGGAGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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