miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22182 5' -58.1 NC_004927.1 + 43123 0.68 0.543956
Target:  5'- -cUUCGUCUUCAUCGAccuccacgGGGaGCGGGACa -3'
miRNA:   3'- ucGAGCAGGAGUGGCU--------CUC-CGCUCUG- -5'
22182 5' -58.1 NC_004927.1 + 22791 0.68 0.543956
Target:  5'- cGGUUUGaCCgcucgUACCGAGAGcGCGGGAg -3'
miRNA:   3'- -UCGAGCaGGa----GUGGCUCUC-CGCUCUg -5'
22182 5' -58.1 NC_004927.1 + 20769 0.69 0.445029
Target:  5'- cGCUUGUCCUCACUcuauuAGAcGUGGGACu -3'
miRNA:   3'- uCGAGCAGGAGUGGc----UCUcCGCUCUG- -5'
22182 5' -58.1 NC_004927.1 + 18578 0.69 0.445029
Target:  5'- gAGCUaCGUCCccaaaaacCACCGcGAGGCGGuGGCu -3'
miRNA:   3'- -UCGA-GCAGGa-------GUGGCuCUCCGCU-CUG- -5'
22182 5' -58.1 NC_004927.1 + 64943 0.71 0.348891
Target:  5'- cAGCUC-UCCagcaUCACCcucGAGGGGCaGAGACg -3'
miRNA:   3'- -UCGAGcAGG----AGUGG---CUCUCCG-CUCUG- -5'
22182 5' -58.1 NC_004927.1 + 53555 0.71 0.356996
Target:  5'- gAGCUgGUCgUgACCG-GGGGCGAcGACu -3'
miRNA:   3'- -UCGAgCAGgAgUGGCuCUCCGCU-CUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.