miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22184 5' -57.1 NC_004927.1 + 32178 0.74 0.288645
Target:  5'- aCGAGGCCGgUCUUGucggugucuUCGUACCCUUCGa -3'
miRNA:   3'- -GUUCCGGUgGGAGU---------AGCAUGGGAGGC- -5'
22184 5' -57.1 NC_004927.1 + 72316 0.74 0.295707
Target:  5'- aGAGcGCCACCCUCGaugCGUACCUaCCc -3'
miRNA:   3'- gUUC-CGGUGGGAGUa--GCAUGGGaGGc -5'
22184 5' -57.1 NC_004927.1 + 72394 0.68 0.586536
Target:  5'- -cGGGCgACCCggacuccgcUCGUgGUgaagACCCUCCGa -3'
miRNA:   3'- guUCCGgUGGG---------AGUAgCA----UGGGAGGC- -5'
22184 5' -57.1 NC_004927.1 + 3304 0.67 0.618293
Target:  5'- gUAAGGCCggaGCCUUCGUau--UCCUCCGg -3'
miRNA:   3'- -GUUCCGG---UGGGAGUAgcauGGGAGGC- -5'
22184 5' -57.1 NC_004927.1 + 21451 0.67 0.65015
Target:  5'- cCGAGGCuCACCgg-GUCGUcGCCCUCaCGg -3'
miRNA:   3'- -GUUCCG-GUGGgagUAGCA-UGGGAG-GC- -5'
22184 5' -57.1 NC_004927.1 + 43889 0.66 0.702807
Target:  5'- gAAGGCCcacuAUCCUCAUCcacGUuCCCUuuGa -3'
miRNA:   3'- gUUCCGG----UGGGAGUAG---CAuGGGAggC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.