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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22187 | 5' | -57.8 | NC_004927.1 | + | 25524 | 0.68 | 0.593263 |
|
Target: 5'- aCCACGucuuCUUCGcCGcCCUCGgagaGCGGGa -3' miRNA: 3'- -GGUGUu---GAGGC-GCuGGAGCa---CGCCCa -5' |
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| 22187 | 5' | -57.8 | NC_004927.1 | + | 35189 | 0.68 | 0.593263 |
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Target: 5'- gCGuCGGCUcCCGUGAaguCCUCG-GCGGGg -3' miRNA: 3'- gGU-GUUGA-GGCGCU---GGAGCaCGCCCa -5' |
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| 22187 | 5' | -57.8 | NC_004927.1 | + | 38673 | 0.67 | 0.645567 |
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Target: 5'- -----uUUCCGCGACCUCGgcagaGCaGGGUu -3' miRNA: 3'- gguguuGAGGCGCUGGAGCa----CG-CCCA- -5' |
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| 22187 | 5' | -57.8 | NC_004927.1 | + | 51181 | 0.68 | 0.593263 |
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Target: 5'- cCCACAAUUCCGUGugCggauuaGU-CGGGa -3' miRNA: 3'- -GGUGUUGAGGCGCugGag----CAcGCCCa -5' |
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| 22187 | 5' | -57.8 | NC_004927.1 | + | 56145 | 0.69 | 0.531541 |
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Target: 5'- -gGCGGCUcCCGaCGAcCCUCGUGUGuGGa -3' miRNA: 3'- ggUGUUGA-GGC-GCU-GGAGCACGC-CCa -5' |
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| 22187 | 5' | -57.8 | NC_004927.1 | + | 65821 | 0.66 | 0.687255 |
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Target: 5'- aCCGCGuuaUCCGCGACaugaaGCGGGa -3' miRNA: 3'- -GGUGUug-AGGCGCUGgagcaCGCCCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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