Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22189 | 3' | -45.8 | NC_004927.1 | + | 14223 | 0.68 | 0.993515 |
Target: 5'- uGAACGUCGUgUCG-CAAUcgGGGCa -3' miRNA: 3'- uCUUGCGGUAgAGCaGUUAuuUCUGg -5' |
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22189 | 3' | -45.8 | NC_004927.1 | + | 69599 | 0.66 | 0.999477 |
Target: 5'- cGAGCGU--UCUCGcaacaaccgcgcUCAGgugGAAGACCu -3' miRNA: 3'- uCUUGCGguAGAGC------------AGUUa--UUUCUGG- -5' |
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22189 | 3' | -45.8 | NC_004927.1 | + | 73445 | 0.66 | 0.999477 |
Target: 5'- gAGGACgGUCAgCUCGUCcgagaGGAGACUg -3' miRNA: 3'- -UCUUG-CGGUaGAGCAGuua--UUUCUGG- -5' |
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22189 | 3' | -45.8 | NC_004927.1 | + | 61829 | 0.66 | 0.99934 |
Target: 5'- cGAACGCUGUCUaucagGUCAcucgcgcuAcGAAGGCCc -3' miRNA: 3'- uCUUGCGGUAGAg----CAGU--------UaUUUCUGG- -5' |
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22189 | 3' | -45.8 | NC_004927.1 | + | 9424 | 0.67 | 0.998732 |
Target: 5'- uAGAA-GCCGUUaUCG-CAAUGGuAGACCa -3' miRNA: 3'- -UCUUgCGGUAG-AGCaGUUAUU-UCUGG- -5' |
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22189 | 3' | -45.8 | NC_004927.1 | + | 3327 | 0.67 | 0.998732 |
Target: 5'- --uGCGCUucucgauuucCUCGUCAGUAAGG-CCg -3' miRNA: 3'- ucuUGCGGua--------GAGCAGUUAUUUCuGG- -5' |
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22189 | 3' | -45.8 | NC_004927.1 | + | 36795 | 0.67 | 0.998102 |
Target: 5'- cGGAACGUccuucucaauCAUCUCGaug--AGAGGCCa -3' miRNA: 3'- -UCUUGCG----------GUAGAGCaguuaUUUCUGG- -5' |
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22189 | 3' | -45.8 | NC_004927.1 | + | 52741 | 0.67 | 0.997699 |
Target: 5'- cGAugGCGaCCAUcCUCGggugCGAgguUGAAGACCu -3' miRNA: 3'- uCU--UGC-GGUA-GAGCa---GUU---AUUUCUGG- -5' |
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22189 | 3' | -45.8 | NC_004927.1 | + | 26306 | 0.68 | 0.996042 |
Target: 5'- uGAACGUCGUagagccaCGUCAGcgGGAGACUg -3' miRNA: 3'- uCUUGCGGUAga-----GCAGUUa-UUUCUGG- -5' |
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22189 | 3' | -45.8 | NC_004927.1 | + | 23245 | 0.68 | 0.994471 |
Target: 5'- gAGAGuCGCCGUCUCcgcUCGGUcggGGAGAgCg -3' miRNA: 3'- -UCUU-GCGGUAGAGc--AGUUA---UUUCUgG- -5' |
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22189 | 3' | -45.8 | NC_004927.1 | + | 21332 | 0.8 | 0.619189 |
Target: 5'- cGGAGCGCCGUUgUCGUCGGUGgcGAaCCa -3' miRNA: 3'- -UCUUGCGGUAG-AGCAGUUAUuuCU-GG- -5' |
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22189 | 3' | -45.8 | NC_004927.1 | + | 10932 | 0.68 | 0.993515 |
Target: 5'- cAGGACucgcccuCCGUCUCGUCGGggAGGGAgCa -3' miRNA: 3'- -UCUUGc------GGUAGAGCAGUUa-UUUCUgG- -5' |
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22189 | 3' | -45.8 | NC_004927.1 | + | 22186 | 0.68 | 0.993515 |
Target: 5'- cGGGGuCGCCGuUCUCGUC-GUGGGGAa- -3' miRNA: 3'- -UCUU-GCGGU-AGAGCAGuUAUUUCUgg -5' |
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22189 | 3' | -45.8 | NC_004927.1 | + | 64554 | 0.69 | 0.99243 |
Target: 5'- gAGAACaGCaacaccaucaAUCUCGUUGAUGGAGAgCu -3' miRNA: 3'- -UCUUG-CGg---------UAGAGCAGUUAUUUCUgG- -5' |
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22189 | 3' | -45.8 | NC_004927.1 | + | 72385 | 0.69 | 0.99243 |
Target: 5'- cGGACuCCG-CUCGU-GGUGAAGACCc -3' miRNA: 3'- uCUUGcGGUaGAGCAgUUAUUUCUGG- -5' |
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22189 | 3' | -45.8 | NC_004927.1 | + | 24171 | 0.7 | 0.984682 |
Target: 5'- gGGAACGUCGaa--GUCGAUGAAGAUa -3' miRNA: 3'- -UCUUGCGGUagagCAGUUAUUUCUGg -5' |
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22189 | 3' | -45.8 | NC_004927.1 | + | 39502 | 0.7 | 0.982805 |
Target: 5'- gGGAGCGCCGUCgaacaaaucugguaGUCAAaGAGcuucGACCg -3' miRNA: 3'- -UCUUGCGGUAGag------------CAGUUaUUU----CUGG- -5' |
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22189 | 3' | -45.8 | NC_004927.1 | + | 68756 | 0.7 | 0.982586 |
Target: 5'- uGAACGCCGcUgUCGaUGAUGAGGACg -3' miRNA: 3'- uCUUGCGGU-AgAGCaGUUAUUUCUGg -5' |
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22189 | 3' | -45.8 | NC_004927.1 | + | 45625 | 0.74 | 0.893555 |
Target: 5'- cGGAUGCCuaccagCUUGcccUCGAUGAAGGCCg -3' miRNA: 3'- uCUUGCGGua----GAGC---AGUUAUUUCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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