Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22190 | 3' | -61.8 | NC_004927.1 | + | 19860 | 0.66 | 0.477984 |
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Target: 5'- aGCaGAuaACCGCCG-CUggCGCGGCUCa -3' miRNA: 3'- cCGaCU--UGGCGGCuGGa-GCGCCGGGg -5' |
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| 22190 | 3' | -61.8 | NC_004927.1 | + | 42513 | 0.67 | 0.420701 |
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Target: 5'- cGGCUGAAucggucgcgggUCGCCggacugauuuuuguGACCUCGCccGUCCCc -3' miRNA: 3'- -CCGACUU-----------GGCGG--------------CUGGAGCGc-CGGGG- -5' |
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| 22190 | 3' | -61.8 | NC_004927.1 | + | 35794 | 0.68 | 0.405965 |
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Target: 5'- aGGCcGAGCCGcCCGACCgagcuacgucCGUGGaggacgCCCa -3' miRNA: 3'- -CCGaCUUGGC-GGCUGGa---------GCGCCg-----GGG- -5' |
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| 22190 | 3' | -61.8 | NC_004927.1 | + | 24130 | 0.69 | 0.348837 |
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Target: 5'- uGUUGuccCCGUCGuCCgUGUGGCCCCg -3' miRNA: 3'- cCGACuu-GGCGGCuGGaGCGCCGGGG- -5' |
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| 22190 | 3' | -61.8 | NC_004927.1 | + | 71951 | 0.73 | 0.197983 |
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Target: 5'- gGGCgcgGAGCCGCUGACgacuaCUCcuaCGGCCUCa -3' miRNA: 3'- -CCGa--CUUGGCGGCUG-----GAGc--GCCGGGG- -5' |
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| 22190 | 3' | -61.8 | NC_004927.1 | + | 70367 | 0.74 | 0.170419 |
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Target: 5'- -aUUGAGCCGgauUCGAUgCUUGCGGCCCCa -3' miRNA: 3'- ccGACUUGGC---GGCUG-GAGCGCCGGGG- -5' |
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| 22190 | 3' | -61.8 | NC_004927.1 | + | 70651 | 0.76 | 0.110105 |
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Target: 5'- cGCUgGAGCCGCCGGgUggUgGUGGCCCCg -3' miRNA: 3'- cCGA-CUUGGCGGCUgG--AgCGCCGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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