miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22191 3' -53.3 NC_004927.1 + 26823 0.66 0.878075
Target:  5'- cCCUUCuuGGCGUCAAcUCGAcccGCcgCCUUCg -3'
miRNA:   3'- -GGAAG--CCGCAGUUcGGUU---CGa-GGAAG- -5'
22191 3' -53.3 NC_004927.1 + 4521 0.66 0.878075
Target:  5'- gCCaUCGGUGUCAcccgguAGCUcgugcuaccagAAGCUCCg-- -3'
miRNA:   3'- -GGaAGCCGCAGU------UCGG-----------UUCGAGGaag -5'
22191 3' -53.3 NC_004927.1 + 32755 0.66 0.862303
Target:  5'- --gUCGGCGUUGAcaCCGcGCUCUUUCa -3'
miRNA:   3'- ggaAGCCGCAGUUc-GGUuCGAGGAAG- -5'
22191 3' -53.3 NC_004927.1 + 24680 0.68 0.790381
Target:  5'- aCCUccCGGCGUCcugccGCCGAggacgaccGCUCCUUg -3'
miRNA:   3'- -GGAa-GCCGCAGuu---CGGUU--------CGAGGAAg -5'
22191 3' -53.3 NC_004927.1 + 73903 0.68 0.78055
Target:  5'- ---gCGGCGaUGAGCCGAGCUUCg-- -3'
miRNA:   3'- ggaaGCCGCaGUUCGGUUCGAGGaag -5'
22191 3' -53.3 NC_004927.1 + 28429 0.69 0.729329
Target:  5'- cCCUucUCGGUGUgguaaGAGCCGGGCgUCCg-- -3'
miRNA:   3'- -GGA--AGCCGCAg----UUCGGUUCG-AGGaag -5'
22191 3' -53.3 NC_004927.1 + 24598 0.69 0.708088
Target:  5'- ---cUGGUGUCAAGuCCAccAGCUCCUc- -3'
miRNA:   3'- ggaaGCCGCAGUUC-GGU--UCGAGGAag -5'
22191 3' -53.3 NC_004927.1 + 49561 0.7 0.664809
Target:  5'- aCC-UCGGCG-UAAGCCGGGgUUCUUUu -3'
miRNA:   3'- -GGaAGCCGCaGUUCGGUUCgAGGAAG- -5'
22191 3' -53.3 NC_004927.1 + 34219 0.72 0.566608
Target:  5'- cCCUUCGGCGUgccgucaguccaCGAGUagucuuCAAGCUCUUUUc -3'
miRNA:   3'- -GGAAGCCGCA------------GUUCG------GUUCGAGGAAG- -5'
22191 3' -53.3 NC_004927.1 + 67776 0.72 0.534511
Target:  5'- uCCcUCGGCGUCGGG-UGAGCUUgUUCa -3'
miRNA:   3'- -GGaAGCCGCAGUUCgGUUCGAGgAAG- -5'
22191 3' -53.3 NC_004927.1 + 29214 0.75 0.3869
Target:  5'- uCCUUCGGCuUCGuagAGCCAucaGGCUCCg-- -3'
miRNA:   3'- -GGAAGCCGcAGU---UCGGU---UCGAGGaag -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.