miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22194 3' -54.9 NC_004927.1 + 8168 0.76 0.259799
Target:  5'- cACACCGGAUGAACCGGGucguCgGGCUUcagUCg -3'
miRNA:   3'- -UGUGGUCUACUUGGUCU----GgCCGAG---AG- -5'
22194 3' -54.9 NC_004927.1 + 22012 0.76 0.273258
Target:  5'- uACACCAGGUagaGAGCCuacauCCGGUUCUCa -3'
miRNA:   3'- -UGUGGUCUA---CUUGGucu--GGCCGAGAG- -5'
22194 3' -54.9 NC_004927.1 + 56382 0.73 0.419472
Target:  5'- aGCcCgCAGA--GACCAGACCGGCUCg- -3'
miRNA:   3'- -UGuG-GUCUacUUGGUCUGGCCGAGag -5'
22194 3' -54.9 NC_004927.1 + 26741 0.72 0.448063
Target:  5'- cGCACuCGGGa----CAGACCGGCUCUCc -3'
miRNA:   3'- -UGUG-GUCUacuugGUCUGGCCGAGAG- -5'
22194 3' -54.9 NC_004927.1 + 47456 0.68 0.680143
Target:  5'- aACACCAGAUGAAgaCGcuCCGGUUCa- -3'
miRNA:   3'- -UGUGGUCUACUUg-GUcuGGCCGAGag -5'
22194 3' -54.9 NC_004927.1 + 56682 0.67 0.76413
Target:  5'- uCACCGGAUGAuuuACCuccccGACgCGGUUCa- -3'
miRNA:   3'- uGUGGUCUACU---UGGu----CUG-GCCGAGag -5'
22194 3' -54.9 NC_004927.1 + 1346 0.66 0.774147
Target:  5'- aACACCGGGaaacacguucgGGGCCGGGCagGGUUUUCa -3'
miRNA:   3'- -UGUGGUCUa----------CUUGGUCUGg-CCGAGAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.