miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22194 5' -46.6 NC_004927.1 + 3974 0.69 0.982548
Target:  5'- aAGGAGuguGGCUucuucauaggcAGUCUGAGuAUCCGCa- -3'
miRNA:   3'- -UCCUC---UCGA-----------UUAGACUUuUAGGUGcu -5'
22194 5' -46.6 NC_004927.1 + 14602 0.71 0.937922
Target:  5'- cGuGGGAGC-GGUCUGGAGcguUCCACGGc -3'
miRNA:   3'- uC-CUCUCGaUUAGACUUUu--AGGUGCU- -5'
22194 5' -46.6 NC_004927.1 + 34395 0.66 0.997251
Target:  5'- cGGuGAGCUGGUCgcUGAGGucGUCCAg-- -3'
miRNA:   3'- uCCuCUCGAUUAG--ACUUU--UAGGUgcu -5'
22194 5' -46.6 NC_004927.1 + 52609 0.69 0.980227
Target:  5'- cGGGAGAGgaAAUCUcGAAAaucGUCCA-GAa -3'
miRNA:   3'- -UCCUCUCgaUUAGA-CUUU---UAGGUgCU- -5'
22194 5' -46.6 NC_004927.1 + 63490 0.66 0.998086
Target:  5'- cGGAGAGCUGA-CgGAaguggggacguugAAAUCUACGc -3'
miRNA:   3'- uCCUCUCGAUUaGaCU-------------UUUAGGUGCu -5'
22194 5' -46.6 NC_004927.1 + 67290 0.67 0.992447
Target:  5'- gAGGAGGGCaagGAgacgGAAGAccgcaucaUCCACGAu -3'
miRNA:   3'- -UCCUCUCGa--UUaga-CUUUU--------AGGUGCU- -5'
22194 5' -46.6 NC_004927.1 + 75235 0.72 0.91387
Target:  5'- cGGGGAGCUGGUC-GAGccAAUCCGgguCGAu -3'
miRNA:   3'- uCCUCUCGAUUAGaCUU--UUAGGU---GCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.