Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
222 | 3' | -55.6 | AC_000008.1 | + | 4224 | 0.66 | 0.585339 |
Target: 5'- gGAGGUAGcACCacUGCA-GAGCUUCaUGCu -3' miRNA: 3'- gCUCCAUU-UGG--ACGUcCUCGAGG-GCG- -5' |
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222 | 3' | -55.6 | AC_000008.1 | + | 13335 | 0.66 | 0.556262 |
Target: 5'- uGAuGGUGcgcAGCCUGUGGagugaaaacuucaccGAGCUgCCGCg -3' miRNA: 3'- gCU-CCAU---UUGGACGUC---------------CUCGAgGGCG- -5' |
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222 | 3' | -55.6 | AC_000008.1 | + | 8343 | 0.67 | 0.529802 |
Target: 5'- aGAuGG-GAGCUguccaugGUcuGGAGCUCCCGCg -3' miRNA: 3'- gCU-CCaUUUGGa------CGu-CCUCGAGGGCG- -5' |
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222 | 3' | -55.6 | AC_000008.1 | + | 13612 | 0.67 | 0.486805 |
Target: 5'- aGAGGc-GGCgCUGCgaaAGGAaaGCUUCCGCa -3' miRNA: 3'- gCUCCauUUG-GACG---UCCU--CGAGGGCG- -5' |
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222 | 3' | -55.6 | AC_000008.1 | + | 27458 | 0.67 | 0.486805 |
Target: 5'- aGGGGcgcAGCUUGCGGGcGGCUUUCGUc -3' miRNA: 3'- gCUCCau-UUGGACGUCC-UCGAGGGCG- -5' |
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222 | 3' | -55.6 | AC_000008.1 | + | 11343 | 0.67 | 0.476311 |
Target: 5'- gCGAGGgagagGAGCCcgaggagaUGCGGGAucgaaaGUUCCaCGCa -3' miRNA: 3'- -GCUCCa----UUUGG--------ACGUCCU------CGAGG-GCG- -5' |
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222 | 3' | -55.6 | AC_000008.1 | + | 12625 | 0.69 | 0.415893 |
Target: 5'- uGGGGcgAuuCCUGCAGGcGUaCCUGCa -3' miRNA: 3'- gCUCCa-UuuGGACGUCCuCGaGGGCG- -5' |
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222 | 3' | -55.6 | AC_000008.1 | + | 27536 | 0.69 | 0.369284 |
Target: 5'- gCGAGGUAuucagcucaacGACgaGuCGGuGAGCUCCuCGCu -3' miRNA: 3'- -GCUCCAU-----------UUGgaC-GUC-CUCGAGG-GCG- -5' |
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222 | 3' | -55.6 | AC_000008.1 | + | 23254 | 0.7 | 0.343087 |
Target: 5'- -cAGGUAcGCCUGCAGGAaucGC-CCCa- -3' miRNA: 3'- gcUCCAUuUGGACGUCCU---CGaGGGcg -5' |
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222 | 3' | -55.6 | AC_000008.1 | + | 12017 | 0.71 | 0.310285 |
Target: 5'- uGAGcGUGAGCCgGCGGcgcGAGCUCagcgaCCGCg -3' miRNA: 3'- gCUC-CAUUUGGaCGUC---CUCGAG-----GGCG- -5' |
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222 | 3' | -55.6 | AC_000008.1 | + | 8381 | 0.71 | 0.310285 |
Target: 5'- -cAGGUcag---GCGGGAGCUCCUGCa -3' miRNA: 3'- gcUCCAuuuggaCGUCCUCGAGGGCG- -5' |
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222 | 3' | -55.6 | AC_000008.1 | + | 27489 | 1.11 | 0.000385 |
Target: 5'- gCGAGGUAAACCUGCAGGAGCUCCCGCc -3' miRNA: 3'- -GCUCCAUUUGGACGUCCUCGAGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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