Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2222 | 3' | -64.1 | NC_001405.1 | + | 4605 | 0.66 | 0.223577 |
Target: 5'- --gGGCCcacgGGCG-GCGGCCUGG-GCGa -3' miRNA: 3'- ggaCCGG----UCGCgCGUCGGGCCuCGCg -5' |
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2222 | 3' | -64.1 | NC_001405.1 | + | 21890 | 0.66 | 0.217918 |
Target: 5'- --cGGCCGGuCGCG-AGaCUgGGGGCGUa -3' miRNA: 3'- ggaCCGGUC-GCGCgUC-GGgCCUCGCG- -5' |
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2222 | 3' | -64.1 | NC_001405.1 | + | 809 | 0.66 | 0.217918 |
Target: 5'- ---cGCCGGCGCcCGGUucuCCGGAGcCGCc -3' miRNA: 3'- ggacCGGUCGCGcGUCG---GGCCUC-GCG- -5' |
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2222 | 3' | -64.1 | NC_001405.1 | + | 23837 | 0.66 | 0.212382 |
Target: 5'- -aUGGCCGcgggcuggguGUGCGCGGCaCC--AGCGCa -3' miRNA: 3'- ggACCGGU----------CGCGCGUCG-GGccUCGCG- -5' |
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2222 | 3' | -64.1 | NC_001405.1 | + | 28518 | 0.66 | 0.206968 |
Target: 5'- --aGGUguGgGCGCAGCaaCCGGuGaCGCa -3' miRNA: 3'- ggaCCGguCgCGCGUCG--GGCCuC-GCG- -5' |
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2222 | 3' | -64.1 | NC_001405.1 | + | 26526 | 0.66 | 0.206968 |
Target: 5'- --gGGUCGGCGaacagGCAGUgCCGGcGGCGCc -3' miRNA: 3'- ggaCCGGUCGCg----CGUCG-GGCC-UCGCG- -5' |
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2222 | 3' | -64.1 | NC_001405.1 | + | 16329 | 0.66 | 0.206968 |
Target: 5'- --cGGCCGcCGCaGCAGCCgCGGccauuAGUGCu -3' miRNA: 3'- ggaCCGGUcGCG-CGUCGG-GCC-----UCGCG- -5' |
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2222 | 3' | -64.1 | NC_001405.1 | + | 7197 | 0.66 | 0.206968 |
Target: 5'- gCCUGGUaGGCGCaGCAuCCCuuuucuacgGGuAGCGCg -3' miRNA: 3'- -GGACCGgUCGCG-CGUcGGG---------CC-UCGCG- -5' |
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2222 | 3' | -64.1 | NC_001405.1 | + | 16104 | 0.66 | 0.206433 |
Target: 5'- --cGGCCAuucagaccgugguGCGCGgAGCCCGGcGUuauGCu -3' miRNA: 3'- ggaCCGGU-------------CGCGCgUCGGGCCuCG---CG- -5' |
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2222 | 3' | -64.1 | NC_001405.1 | + | 5663 | 0.66 | 0.205367 |
Target: 5'- aCCUGGCUcguGGaaugcgagaccgucUGCGgGGCCUGcGGCGCg -3' miRNA: 3'- -GGACCGG---UC--------------GCGCgUCGGGCcUCGCG- -5' |
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2222 | 3' | -64.1 | NC_001405.1 | + | 23242 | 0.67 | 0.196496 |
Target: 5'- ---aGCCAcaaCGCGCAGCCCGuGGGCu- -3' miRNA: 3'- ggacCGGUc--GCGCGUCGGGC-CUCGcg -5' |
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2222 | 3' | -64.1 | NC_001405.1 | + | 8697 | 0.67 | 0.186491 |
Target: 5'- uCCU-GCCcGCGCGCGGCgCCGacGUGCc -3' miRNA: 3'- -GGAcCGGuCGCGCGUCG-GGCcuCGCG- -5' |
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2222 | 3' | -64.1 | NC_001405.1 | + | 22309 | 0.67 | 0.186491 |
Target: 5'- uCCgcaGCCacAGUGCGCAGauuaGGAGCGCc -3' miRNA: 3'- -GGac-CGG--UCGCGCGUCggg-CCUCGCG- -5' |
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2222 | 3' | -64.1 | NC_001405.1 | + | 12211 | 0.67 | 0.18023 |
Target: 5'- uCC-GGCCccagcugccuccagGGCGCGuCGGCuuGGggcccAGCGCa -3' miRNA: 3'- -GGaCCGG--------------UCGCGC-GUCGggCC-----UCGCG- -5' |
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2222 | 3' | -64.1 | NC_001405.1 | + | 18543 | 0.67 | 0.176936 |
Target: 5'- gCUGGcCCAGCacuccgGUAGCCauGGGCGCg -3' miRNA: 3'- gGACC-GGUCGcg----CGUCGGgcCUCGCG- -5' |
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2222 | 3' | -64.1 | NC_001405.1 | + | 15968 | 0.67 | 0.172325 |
Target: 5'- --cGGCacuacCGCGC-GCCCuGGGGCGCg -3' miRNA: 3'- ggaCCGguc--GCGCGuCGGG-CCUCGCG- -5' |
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2222 | 3' | -64.1 | NC_001405.1 | + | 25361 | 0.68 | 0.156599 |
Target: 5'- aCCUGGCaaacggccaugGGCGUGUGGCagcaaugccuggaGGAGCGCa -3' miRNA: 3'- -GGACCGg----------UCGCGCGUCGgg-----------CCUCGCG- -5' |
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2222 | 3' | -64.1 | NC_001405.1 | + | 11523 | 0.68 | 0.150438 |
Target: 5'- --cGGCCGccacgugugcGCGCGCGGgacuaauCCCGGuccGCGCg -3' miRNA: 3'- ggaCCGGU----------CGCGCGUC-------GGGCCu--CGCG- -5' |
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2222 | 3' | -64.1 | NC_001405.1 | + | 25029 | 0.68 | 0.146848 |
Target: 5'- gCUGGCUugAGaCGCGCgAGCCUgccgacuuggaGGAGCGa -3' miRNA: 3'- gGACCGG--UC-GCGCG-UCGGG-----------CCUCGCg -5' |
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2222 | 3' | -64.1 | NC_001405.1 | + | 16043 | 0.69 | 0.139149 |
Target: 5'- --cGGCCGcguuuGUGCGCGcCCCaGGGCGCg -3' miRNA: 3'- ggaCCGGU-----CGCGCGUcGGGcCUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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