Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2222 | 5' | -54.4 | NC_001405.1 | + | 9537 | 0.66 | 0.685154 |
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Target: 5'- cCCGUcauGuCCCGGuuaUGgGUUGGCGGGGg -3' miRNA: 3'- -GGCA---CuGGGUCug-AUgCGACUGCUCC- -5' |
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| 2222 | 5' | -54.4 | NC_001405.1 | + | 7625 | 0.67 | 0.618148 |
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Target: 5'- cCCGUGuucugacaggGCCCAGuCUGCaa-GAUGAGGg -3' miRNA: 3'- -GGCAC----------UGGGUCuGAUGcgaCUGCUCC- -5' |
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| 2222 | 5' | -54.4 | NC_001405.1 | + | 12894 | 0.69 | 0.534134 |
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Target: 5'- aCC-UGAgCCAGGCUuucaagaacuugcagGgGCUGugGGGGg -3' miRNA: 3'- -GGcACUgGGUCUGA---------------UgCGACugCUCC- -5' |
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| 2222 | 5' | -54.4 | NC_001405.1 | + | 5334 | 0.69 | 0.497792 |
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Target: 5'- -gGUGcGCuCCGGGCUgcGCGCUGGCcAGGg -3' miRNA: 3'- ggCAC-UG-GGUCUGA--UGCGACUGcUCC- -5' |
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| 2222 | 5' | -54.4 | NC_001405.1 | + | 11189 | 0.7 | 0.4455 |
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Target: 5'- gCCG-GGCCCGGcACUAC-CUGgacuuggaggaggGCGAGGg -3' miRNA: 3'- -GGCaCUGGGUC-UGAUGcGAC-------------UGCUCC- -5' |
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| 2222 | 5' | -54.4 | NC_001405.1 | + | 2680 | 0.72 | 0.371001 |
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Target: 5'- aUGUGGCCgGGgguGCUugGCaugGACGGGGu -3' miRNA: 3'- gGCACUGGgUC---UGAugCGa--CUGCUCC- -5' |
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| 2222 | 5' | -54.4 | NC_001405.1 | + | 10369 | 0.72 | 0.345023 |
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Target: 5'- -gGUGGCCgGGGCUcCGggGGCGAGGu -3' miRNA: 3'- ggCACUGGgUCUGAuGCgaCUGCUCC- -5' |
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| 2222 | 5' | -54.4 | NC_001405.1 | + | 5363 | 1.13 | 0.000448 |
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Target: 5'- aCCGUGACCCAGACUACGCUGACGAGGa -3' miRNA: 3'- -GGCACUGGGUCUGAUGCGACUGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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