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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22225 | 5' | -44.4 | NC_005038.1 | + | 79346 | 0.66 | 0.999975 |
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Target: 5'- ---gCGUGUUCg--UAUGUUUGCGAUa -3' miRNA: 3'- aauaGCGCAAGauuGUGCAAAUGCUG- -5' |
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| 22225 | 5' | -44.4 | NC_005038.1 | + | 49211 | 0.67 | 0.999885 |
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Target: 5'- uUUGUCGCGaUUUUu---CGUUUugGACg -3' miRNA: 3'- -AAUAGCGC-AAGAuuguGCAAAugCUG- -5' |
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| 22225 | 5' | -44.4 | NC_005038.1 | + | 7436 | 0.67 | 0.999848 |
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Target: 5'- -gAUCGUGcggaUCUAACACacagGCGACa -3' miRNA: 3'- aaUAGCGCa---AGAUUGUGcaaaUGCUG- -5' |
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| 22225 | 5' | -44.4 | NC_005038.1 | + | 26914 | 0.68 | 0.999573 |
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Target: 5'- aUAUUGCG-UUUGACGCGUg-GCGAg -3' miRNA: 3'- aAUAGCGCaAGAUUGUGCAaaUGCUg -5' |
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| 22225 | 5' | -44.4 | NC_005038.1 | + | 79453 | 0.68 | 0.999317 |
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Target: 5'- uUUGUCGUuaUUUGuguauaauGCAUGUUUACGACa -3' miRNA: 3'- -AAUAGCGcaAGAU--------UGUGCAAAUGCUG- -5' |
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| 22225 | 5' | -44.4 | NC_005038.1 | + | 51569 | 0.77 | 0.875709 |
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Target: 5'- aUAUCGUGcacuacUUCUAACACGUccACGGCa -3' miRNA: 3'- aAUAGCGC------AAGAUUGUGCAaaUGCUG- -5' |
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| 22225 | 5' | -44.4 | NC_005038.1 | + | 54908 | 1.1 | 0.022778 |
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Target: 5'- gUUAUCGCGUUCUAACACGUUUACGACa -3' miRNA: 3'- -AAUAGCGCAAGAUUGUGCAAAUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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