Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2223 | 3' | -51.5 | NC_001405.1 | + | 25248 | 0.66 | 0.812231 |
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Target: 5'- aCCUUGGAAuUUUGCACgaaaaCCgCCUCGg -3' miRNA: 3'- gGGAAUUUUcAGACGUGac---GG-GGAGC- -5' |
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| 2223 | 3' | -51.5 | NC_001405.1 | + | 24013 | 0.66 | 0.791918 |
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Target: 5'- gCUCggggguGGUUUcGCGCUGCUCCUCu -3' miRNA: 3'- -GGGaauuu-UCAGA-CGUGACGGGGAGc -5' |
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| 2223 | 3' | -51.5 | NC_001405.1 | + | 1402 | 0.66 | 0.770857 |
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Target: 5'- aCCCggu--GGUCccGCugUGCCCCa-- -3' miRNA: 3'- -GGGaauuuUCAGa-CGugACGGGGagc -5' |
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| 2223 | 3' | -51.5 | NC_001405.1 | + | 11140 | 0.67 | 0.76008 |
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Target: 5'- gCCCUgcau-GUCUGcCGCUGCUCUUg- -3' miRNA: 3'- -GGGAauuuuCAGAC-GUGACGGGGAgc -5' |
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| 2223 | 3' | -51.5 | NC_001405.1 | + | 25859 | 0.68 | 0.658094 |
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Target: 5'- aCCUUu-GAG-CUGCAggGUCCCUCGc -3' miRNA: 3'- gGGAAuuUUCaGACGUgaCGGGGAGC- -5' |
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| 2223 | 3' | -51.5 | NC_001405.1 | + | 5573 | 1.11 | 0.001038 |
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Target: 5'- gCCCUUAAAAGUCUGCACUGCCCCUCGu -3' miRNA: 3'- -GGGAAUUUUCAGACGUGACGGGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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