Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22234 | 3' | -55.9 | NC_005045.1 | + | 33680 | 0.66 | 0.626746 |
Target: 5'- cCUGCCGCCgACca-UCAAgGCCCUgGc -3' miRNA: 3'- -GACGGCGGgUGcacAGUUaUGGGAgC- -5' |
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22234 | 3' | -55.9 | NC_005045.1 | + | 39632 | 0.66 | 0.626746 |
Target: 5'- cCUGCCGgCgACGgcggcgacgagcUGUCGuAUGCCCUgGg -3' miRNA: 3'- -GACGGCgGgUGC------------ACAGU-UAUGGGAgC- -5' |
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22234 | 3' | -55.9 | NC_005045.1 | + | 9414 | 0.66 | 0.593386 |
Target: 5'- aCUGCCGaCCGgG-GUCAAgGCCCUg- -3' miRNA: 3'- -GACGGCgGGUgCaCAGUUaUGGGAgc -5' |
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22234 | 3' | -55.9 | NC_005045.1 | + | 14211 | 0.66 | 0.593386 |
Target: 5'- -aGCgGCCCAUGg--CGGUGCCCgcCGg -3' miRNA: 3'- gaCGgCGGGUGCacaGUUAUGGGa-GC- -5' |
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22234 | 3' | -55.9 | NC_005045.1 | + | 23021 | 0.67 | 0.517106 |
Target: 5'- -gGCUGCCCAaauCGUGUCcAUcAUCUUCGg -3' miRNA: 3'- gaCGGCGGGU---GCACAGuUA-UGGGAGC- -5' |
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22234 | 3' | -55.9 | NC_005045.1 | + | 2173 | 0.68 | 0.506503 |
Target: 5'- -aGCaGCCC-CGcGUCGAUACCCUg- -3' miRNA: 3'- gaCGgCGGGuGCaCAGUUAUGGGAgc -5' |
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22234 | 3' | -55.9 | NC_005045.1 | + | 9477 | 0.69 | 0.425502 |
Target: 5'- -cGUCGCCCugGccgGUgGGUuCCCUCGc -3' miRNA: 3'- gaCGGCGGGugCa--CAgUUAuGGGAGC- -5' |
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22234 | 3' | -55.9 | NC_005045.1 | + | 39361 | 0.7 | 0.387994 |
Target: 5'- -aGCCGCCCagccACGggugccGUCGAgGCCCUUGc -3' miRNA: 3'- gaCGGCGGG----UGCa-----CAGUUaUGGGAGC- -5' |
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22234 | 3' | -55.9 | NC_005045.1 | + | 18186 | 0.7 | 0.387994 |
Target: 5'- aUGCgUGCCCugGggaugcgGUCGAUggACCCUUGg -3' miRNA: 3'- gACG-GCGGGugCa------CAGUUA--UGGGAGC- -5' |
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22234 | 3' | -55.9 | NC_005045.1 | + | 22765 | 0.7 | 0.364808 |
Target: 5'- gUGCCGCCCAgCGUGUucucugccucuuccgCAGUGaugcggacuUCCUCGg -3' miRNA: 3'- gACGGCGGGU-GCACA---------------GUUAU---------GGGAGC- -5' |
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22234 | 3' | -55.9 | NC_005045.1 | + | 7681 | 0.71 | 0.352725 |
Target: 5'- cCUGgCGUcaUCACGUuguaGUCGAUGCCCUUGg -3' miRNA: 3'- -GACgGCG--GGUGCA----CAGUUAUGGGAGC- -5' |
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22234 | 3' | -55.9 | NC_005045.1 | + | 13881 | 0.72 | 0.289209 |
Target: 5'- gCUGCCGUCCGgGUgguccacgaacGUCAGgguuuCCCUCGg -3' miRNA: 3'- -GACGGCGGGUgCA-----------CAGUUau---GGGAGC- -5' |
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22234 | 3' | -55.9 | NC_005045.1 | + | 962 | 1.08 | 0.000751 |
Target: 5'- cCUGCCGCCCACGUGUCAAUACCCUCGc -3' miRNA: 3'- -GACGGCGGGUGCACAGUUAUGGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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