miRNA: 3'- gagugaUCCAGACAGaGAACUuaGGG- -5'
miRNA: 3'- gAGU-GAUCCAGACAGAGaac--UUAGGg -5'
miRNA: 3'- -GAGUGAUCCAGACAGAGAACUUAGGG- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22235 | 3' | -48.7 | NC_005045.1 | + | 1680 | 0.66 | 0.954799 |
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Target: 5'- -----aAGGUUUGUCcCUUGAggCCCu -3' miRNA: 3'- gagugaUCCAGACAGaGAACUuaGGG- -5' |
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| 22235 | 3' | -48.7 | NC_005045.1 | + | 38037 | 0.72 | 0.672702 |
|
Target: 5'- aUCGuCUAGGUCUGUCUUgauauAGUCCa -3' miRNA: 3'- gAGU-GAUCCAGACAGAGaac--UUAGGg -5' |
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| 22235 | 3' | -48.7 | NC_005045.1 | + | 1778 | 1.13 | 0.002094 |
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Target: 5'- cCUCACUAGGUCUGUCUCUUGAAUCCCc -3' miRNA: 3'- -GAGUGAUCCAGACAGAGAACUUAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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