miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22237 5' -56.1 NC_005045.1 + 3333 1.1 0.000428
Target:  5'- cGUCAUGCACCGCGACGGCUUGGUCACa -3'
miRNA:   3'- -CAGUACGUGGCGCUGCCGAACCAGUG- -5'
22237 5' -56.1 NC_005045.1 + 22001 0.7 0.336721
Target:  5'- cUCugcUGUACCGCGACaGCgacgccgcuacggUGGUCGCa -3'
miRNA:   3'- cAGu--ACGUGGCGCUGcCGa------------ACCAGUG- -5'
22237 5' -56.1 NC_005045.1 + 40570 0.68 0.451081
Target:  5'- ---cUGCGCCGCGcaGgGGCUUGuUCACu -3'
miRNA:   3'- caguACGUGGCGC--UgCCGAACcAGUG- -5'
22237 5' -56.1 NC_005045.1 + 12270 0.67 0.524282
Target:  5'- cGUgAUG-ACCGgGcCGuGCUUGGUCGCc -3'
miRNA:   3'- -CAgUACgUGGCgCuGC-CGAACCAGUG- -5'
22237 5' -56.1 NC_005045.1 + 5977 0.66 0.568103
Target:  5'- uUCcgGCAUCGCGcagggcACGGUuuaccgccagcUUGGUUACg -3'
miRNA:   3'- cAGuaCGUGGCGC------UGCCG-----------AACCAGUG- -5'
22237 5' -56.1 NC_005045.1 + 41691 0.66 0.568103
Target:  5'- cUCGgugaugGCGCCGU--UGGCUaUGGUCACc -3'
miRNA:   3'- cAGUa-----CGUGGCGcuGCCGA-ACCAGUG- -5'
22237 5' -56.1 NC_005045.1 + 30741 0.66 0.590371
Target:  5'- aUCAgugGCGCgcuguCGCGGCGGCUgaacaGGUCc- -3'
miRNA:   3'- cAGUa--CGUG-----GCGCUGCCGAa----CCAGug -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.