Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22238 | 3' | -47.9 | NC_005045.1 | + | 3524 | 1.15 | 0.001752 |
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Target: 5'- cGCCUUGCGUGAGUAGAAUCGCAAUGGa -3' miRNA: 3'- -CGGAACGCACUCAUCUUAGCGUUACC- -5' |
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| 22238 | 3' | -47.9 | NC_005045.1 | + | 15579 | 0.73 | 0.709275 |
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Target: 5'- gGCg-UGCGUGAGcAGGAUCGaUggUGGa -3' miRNA: 3'- -CGgaACGCACUCaUCUUAGC-GuuACC- -5' |
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| 22238 | 3' | -47.9 | NC_005045.1 | + | 41331 | 0.69 | 0.888875 |
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Target: 5'- aGCC-UGgGUG-GUAGggUCaauCAGUGGg -3' miRNA: 3'- -CGGaACgCACuCAUCuuAGc--GUUACC- -5' |
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| 22238 | 3' | -47.9 | NC_005045.1 | + | 3422 | 0.67 | 0.956003 |
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Target: 5'- uGCCUU-CGU--GUGGAGUCGCAAg-- -3' miRNA: 3'- -CGGAAcGCAcuCAUCUUAGCGUUacc -5' |
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| 22238 | 3' | -47.9 | NC_005045.1 | + | 20352 | 0.66 | 0.970947 |
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Target: 5'- gGCCccUGCGUGcucGUAGAGcuggcgaaacagcUCGgGGUGGa -3' miRNA: 3'- -CGGa-ACGCACu--CAUCUU-------------AGCgUUACC- -5' |
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| 22238 | 3' | -47.9 | NC_005045.1 | + | 2259 | 0.66 | 0.971275 |
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Target: 5'- aGCCggcacUGCGUgGAGUGGucaggguAUCGaCGcgGGg -3' miRNA: 3'- -CGGa----ACGCA-CUCAUCu------UAGC-GUuaCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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