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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 22238 | 5' | -54.8 | NC_005045.1 | + | 3452 | 0.66 | 0.670438 |
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Target: 5'- -gCGUccaUUGCGAUUCUACucACGcAAGGCGg -3' miRNA: 3'- caGCG---AACGCUGAGGUG--UGC-UUCCGU- -5' |
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| 22238 | 5' | -54.8 | NC_005045.1 | + | 21298 | 0.66 | 0.670438 |
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Target: 5'- aUCGCUUucacGaUGGCUCCagcACACGAGGGgGa -3' miRNA: 3'- cAGCGAA----C-GCUGAGG---UGUGCUUCCgU- -5' |
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| 22238 | 5' | -54.8 | NC_005045.1 | + | 38463 | 0.67 | 0.591243 |
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Target: 5'- -cCGCcUGCGccugccgcauCUCCuCGCGGAGGCGg -3' miRNA: 3'- caGCGaACGCu---------GAGGuGUGCUUCCGU- -5' |
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| 22238 | 5' | -54.8 | NC_005045.1 | + | 21969 | 0.68 | 0.513764 |
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Target: 5'- uGUUGCuUUGCGA-UCCACcCGAgccaAGGCAg -3' miRNA: 3'- -CAGCG-AACGCUgAGGUGuGCU----UCCGU- -5' |
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| 22238 | 5' | -54.8 | NC_005045.1 | + | 23677 | 0.69 | 0.471359 |
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Target: 5'- aUCGCgguucucgGCGGCUuuGC-CGAAGGCu -3' miRNA: 3'- cAGCGaa------CGCUGAggUGuGCUUCCGu -5' |
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| 22238 | 5' | -54.8 | NC_005045.1 | + | 3490 | 1.08 | 0.000872 |
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Target: 5'- gGUCGCUUGCGACUCCACACGAAGGCAc -3' miRNA: 3'- -CAGCGAACGCUGAGGUGUGCUUCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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