Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22239 | 3' | -55.2 | NC_005045.1 | + | 33016 | 0.69 | 0.434845 |
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Target: 5'- aGGAgcugGUaGCCugGGGCCUGAc----- -3' miRNA: 3'- gCCUa---CA-CGGugCCCGGACUucaacg -5' |
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| 22239 | 3' | -55.2 | NC_005045.1 | + | 29751 | 0.68 | 0.503499 |
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Target: 5'- gCGGGUG-GCCaACGGuGCCaucacagaccucgcUGAGGUUGa -3' miRNA: 3'- -GCCUACaCGG-UGCC-CGG--------------ACUUCAACg -5' |
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| 22239 | 3' | -55.2 | NC_005045.1 | + | 20090 | 0.68 | 0.517375 |
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Target: 5'- gCGGGUccgGCUGCGGGCC-GAGGcUGUc -3' miRNA: 3'- -GCCUAca-CGGUGCCCGGaCUUCaACG- -5' |
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| 22239 | 3' | -55.2 | NC_005045.1 | + | 33733 | 0.67 | 0.605463 |
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Target: 5'- -uGAUGgGCCaACGGGCCaggGAAGcaggugUGCg -3' miRNA: 3'- gcCUACaCGG-UGCCCGGa--CUUCa-----ACG- -5' |
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| 22239 | 3' | -55.2 | NC_005045.1 | + | 11853 | 0.66 | 0.639131 |
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Target: 5'- uGGAgUGUGCC---GGCCUGAAGgUGg -3' miRNA: 3'- gCCU-ACACGGugcCCGGACUUCaACg -5' |
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| 22239 | 3' | -55.2 | NC_005045.1 | + | 39027 | 0.66 | 0.66156 |
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Target: 5'- aGGAUGUcaGCC-CGGuagcCCUGGAGggcgGCg -3' miRNA: 3'- gCCUACA--CGGuGCCc---GGACUUCaa--CG- -5' |
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| 22239 | 3' | -55.2 | NC_005045.1 | + | 11843 | 0.72 | 0.303019 |
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Target: 5'- -----aUGCCACGGGCCUGAAGc--- -3' miRNA: 3'- gccuacACGGUGCCCGGACUUCaacg -5' |
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| 22239 | 3' | -55.2 | NC_005045.1 | + | 14811 | 0.75 | 0.209782 |
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Target: 5'- aGGAUGUGCa--GGGCCgagccgggGAAGUUGg -3' miRNA: 3'- gCCUACACGgugCCCGGa-------CUUCAACg -5' |
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| 22239 | 3' | -55.2 | NC_005045.1 | + | 3941 | 1.11 | 0.000509 |
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Target: 5'- cCGGAUGUGCCACGGGCCUGAAGUUGCc -3' miRNA: 3'- -GCCUACACGGUGCCCGGACUUCAACG- -5' |
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| 22239 | 3' | -55.2 | NC_005045.1 | + | 23603 | 0.66 | 0.66156 |
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Target: 5'- uGGAggGUGCCaacuGCGGGUCgccuucGAGGUuuccUGCc -3' miRNA: 3'- gCCUa-CACGG----UGCCCGGa-----CUUCA----ACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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